摘要
目的探讨三苯氧胺(TAM)对雌激素受体阳性乳腺癌细胞侵袭转移能力的作用及机制。方法用不同浓度(10-7、10-8、10-9mol/L)的TAM作用于人乳腺癌MCF-7细胞株,48h后,用Transwell侵袭小室法检测细胞的侵袭能力,用RT-PCR法检测细胞基质金属蛋白酶-9(MMP-9)和金属蛋白酶组织抑制物-1(TIMP-1)基因的表达。结果上述浓度的TAM对MCF-7侵袭转移均有一定的抑制作用,均可下调其MMP-9基因表达,上调其TIMP-1基因表达,但组间比较没有显著性差异。结论 TAM可抑制雌激素受体阳性MCF-7乳腺癌细胞的侵袭和转移,在10-9mol/L～10-7mol/L之间,没有明显的剂量依赖关系;其作用机制与影响MMP-9/TIMP-1的平衡有关。
Objective To explore the effect of tamoxifen on invasion and metastasis of estrogen peceptor-positive breast cancer cell and its mechanism. Methods TAM with different concentrations（10-7,10-8,10-9 mol/L） were added to human breast cancer cell line MCF-7,after 48 h,the ability of cell invasion was detected by Transwell method,the expression of MMP-9,TIMP-1 was detected by RT-PCR method. Results TAM with above concentrations had certain inhibition on invasion and metastasis of MCF-7,and might downregulate the MMP-9 gene expression,upregulate the TIMP-1 gene expression,but there was no significant difference in these groups. Conclusion TAM can inhibit invasion and metastasis of estrogen receptor-positive MCF-7 breast cancer cell,there is no obvious dose-dependence between 10-9 mol/L and 10-7 mol/L,its mechanism is related with the balance of influencing of MMP-9/TIMP-1.
出处
《临床医学工程》
2010年第10期46-48,共3页
Clinical Medicine & Engineering
