摘要
目的 :分析基质金属蛋白酶 (MMPs)及其组织抑制物 (TIMPs)在滋养细胞疾病中表达水平与预后的关系。方法 :采用免疫组化S P法检测 17例正常绒毛、39例葡萄胎 (HM)、4 2例侵蚀性葡萄胎 (IM)和 2 0例绒毛膜癌 (CC)组织中MMP 2、MMP 9及其TIMP 1、TIMP 2的表达情况。结果 :葡萄胎恶变组MMP 2、MMP 9表达较正常绒毛和葡萄胎未恶变组高 (P =0 .0 0 0 ,0 .0 0 0 ;P =0 .0 4 0 ,0 .0 11)。未化疗滋养细胞肿瘤 (包括IM和CC)MMP 2表达强于早孕绒毛和葡萄胎组 (P均 =0 .0 0 0 ) ,而≥ 3疗程化疗后MMP 2、9表达明显低于未化疗和≤ 2疗程化疗组 (P =0 .0 0 0 ,0 .0 35 ;P =0 .0 10 ,0 .0 18) ,TIMP 1在未化疗滋养细胞肿瘤组表达低于早孕绒毛和葡萄胎组 (P =0 .0 0 5 ,P =0 .0 0 0 )。MMP 2在Ⅱ、Ⅲ期的表达明显强于Ⅰ期 (P均 =0 .0 0 0 ) ,WHO预后评分中、高危者明显强于低危者 (P =0 .0 0 2 ,P =0 .0 0 5 )。结论 :MMPs及TIMPs表达水平高低与滋养细胞疾病预后有关 ,深入了解两者在滋养细胞疾病中的生物作用 。
Objective: To investigate the expression of MMPs and their inhibitors-TIMPs in gestational trophoblastic disease and to assess the relationship between the expression of MMPs and some prognostic factors. Methods: The expression of MMPs and TIMPs was detected by immunohistochemistry in 17 cases of normal villi and 101 cases of trophoblastic disease [39 hydatidiform mole (HM), 42 invasive hydatidiform mole (IM), and 20 choroicarcinomas (CC)]. Results: The expression of MMP-2?MMP-9 was higher in malignant transformed mole than that in normal villi and non-malignant transformed mole (P=0.000, 0.000; P=0.040, 0.011, respectively). MMP-2 expression in trophoblastic tumor (IM+CC) groupswithout chemotherapy was significantly higher than that in the groups of normal villi and hydatidiform mole (P=0.000), while it declined after more than 3 chemotherapeutic regimens and was lower than that in no chemotherapy and less than 2 chemotherapeutic regimens (P=0.000, P=0.035), similar of MMP-9. There was significant difference about TIMP-1 expression between trophoblastic tumor groups without chemotherapy and normal villi?hydatidiform mole (P=0.005, P=0.000). Compared with that of stageⅠ(WHO) , expression of MMP-2 in the stage Ⅱand Ⅲ was significantly increased(P= 0.000,P=0.000). The value of MMP-2 in the cases with middle or high risk in WHO prognostic scoring system was higher than in the cases with low risk (P=0.002, P=0.005). Conclusion: The expression of MMPs and their inhibitors TIMPs has the close relationship with the prognostic factors in gestational trophoblastic disease. To study the biologic function of MMPs and their inhibitors in GTD can help us to find a new method to deal with the drug resistance.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2004年第4期231-233,i009,共4页
Journal of Practical Obstetrics and Gynecology
关键词
基质金属蛋白酶
基质金属蛋白酶抑制物
妊娠滋养细胞疾病
Matrix metalloproteinase
Tissue inhibitors of matrix metalloproteinase
Gestational trophoblastic disease
