摘要
应用蛋白质组学双向凝胶电泳(Two-dimensional gel electrophoresis,2DE)和质谱技术,定量分析和鉴定了癫痫组(n=3)和正常组(n=3)脑组织的差异表达蛋白,以从蛋白质水平上揭示癫痫病的发生机制.结果表明,凝胶图谱可辨识2500～3000个蛋白点,对21个显著差异表达蛋白点进行质谱鉴定和SwissProt数据库检索,得到17个癫痫差异蛋白,其中2个蛋白在癫痫组织中表达上调,15个蛋白表达下调.部分蛋白与癫痫的关系属首次报道.这些蛋白与癫痫的病变相关,可能成为癫痫的分子标志物和药物治疗的靶向蛋白.
To explore the mechanism of epilepsy,the proteins that were differentially expressed in the epilepsy and normal tissue were studied.Two-dimensional gel electrophoresis was performed to separate differentially expressed proteins that were subsequently identified by mass spectrometry.Totally 2500—3000 spots were detected in samples,21 significantly differentially expressed spots were selected,and 17 proteins were identified.Two proteins in epilepsy are up-regulated,15 proteins are down-regulated.Part of the identified protein candidates have been reported to be associated with epilepsy.However,some of the proteins identified in current were for the first time revealed to be linked to epilepsy.The functions of these proteins,the role of them in epilepsy were discussed.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2010年第10期1970-1975,共6页
Chemical Journal of Chinese Universities
基金
江苏省自然科学基金(批准号:BK2009428)
江苏省高校自然科学基础研究项目(批准号:08KJB320010)
南京中医药大学基础研究与重点培育专项基金(批准号:10XPY04)资助
关键词
癫痫
双向凝胶电泳
质谱
蛋白质组学
Epilepsy
Two-dimensional gel electrophoresis
Mass spectrometry
Proteomics
