摘要
目的:建立测定人血浆中舍吲哚浓度的高效液相色谱法。方法:以DiamonsilTMC18柱(150mm×4.6mm,5μm)为色谱柱;流动相为0.03mol.L-1磷酸二氢钾水溶液(调pH6.4)-乙腈(22∶78);流速:0.8mL.min-1;柱温:40℃;检测波长:258nm。以醋酸乙酯与二氯甲烷(80∶20)为提取剂。结果:舍吲哚的高、中、低(320.0,40.0,5.0μg.L-1)3种浓度平均相对回收率分别为98.62%,99.71%,101.82%;提取回收率分别为86.47%,84.32%,79.56%。日内、日间差RSD均低于7%(n=5);分析方法的检测限为5.0μg.L-1;线性范围为5.0～320.0μg.L-1。曲线方程:Y=97.19X+3.16,r=0.999 1(n=7)。结论:该方法灵敏、准确、简单、快速,可用于临床血药浓度监测和药动学研究。
OBJECTIVE To establish a method for determining the concentration of sertindole in human plasma by RPHPLC. METHODS The drug from plasma was analyzed in a reverse phase HPLC system C18 column（151） mm × 4. 6 mm, 5 μm） ; mobile phase consisted of 0. 03 mol · L^-1 Postassium dihydrogen phosphate（pH 6. 4）-Acetonitrile （22.78） ; the flow rate was 0. 8 mL·min^-1 ; the detection wavelength was at 258 nm. Ethyl acetate and dichloromethane（80： 20）was used as extracting solvent. RESULTS The average relative recoveries of sertindole in high, middle and low concentrations （320. 0,40. 0, 5.0 ng · mL^-1 ） were 98.62%, 99. 71% and 101.82%, respectively; the extraction recovery were 86. 47%, 84. 32% and 79. 56%, respectively. The intra-day and inter-day variation（RSD）was less than 7% （n = 5）. The calibration curve of sertindole showed good linearity, r = 0. 9991 （n = 7）, over the range of 5. 0-320. 0 ng·mL^-1. The regression equation was Y = 97. 19X ＋ 3. 16. r = 0. 9991 （n = 7）. The minimum detectable concentration of sertindole was 5.0 ng·mL^-1. CONCLUSION The method is sensitive, accurate, easy and quick, which can be used for clinical drug monitoring and pharmacokinetics studies.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2010年第18期1570-1572,共3页
Chinese Journal of Hospital Pharmacy
基金
广东省自然科学基金资助项目(编号:8151037001000001)
