摘要
Ｍｏｌｅｃｕｌａｒｃｌｏｎｉｎｇｔｅｃｈｎｉｑｕｅｓａｎｄｐｈａｒｍａｃｏ－ｌｏｇｉｃａｌｓｔｕｄｉｅｓｈａｖｅｓｈｏｗｎｔｈａｔα１－ａｄｒｅｎｏｃｅｐｔｏｒｓ（α１－ＡＲ）ａｒｅｄｉｖｉｄｅｄｉｎｔｏｔｈｒｅｅｓｕｂｔｙｐｅｓ，ｔｈａｔｉｓ，α１...
The three α 1 adrenoceptor(α 1 AR) subtypes were compared in renal arteries from 12 month old spontaneously hypertensive rats(SHR) and aged matched WKY rats using in vitro contractile studies and RNase protection assays. Contractile studies showed that norepinephrine (NE) induced contraction, including pD 2 values and maximum contractions, was not significantly different between WKY rats and SHR; pA 2 values of α 1A selective antagonists WB4101 and sertindole were increased from 9.08±0.09 and 8.58±0.13 in WKY rats to 9.50±0.24 and 8.94±0.20 in SHR, respectively; whereas pA 2 value of α 1D selective antagonist BMY7378 was not significantly different between WKY rats and SHR (7.59±0.29 vs 7.23±0.27). The results of RNase protection assays showed that mRNA levels for α 1A , α 1B and α 1D AR subtypes were not significantly different between WKY rats and SHR. The results suggest that the potency of NE in renal artery be not significantly different between WKY rat and SHR, but α 1A subtype make a more contribution to NE induced responses in renal artery from SHR than from WKY rats. The functional change may result from the change in receptor protein and/or post receptor signal transduction.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
1999年第1期9-12,共4页
Chinese Journal of Pharmacology and Toxicology
关键词
高血压
肾动脉
肾上腺素
受体亚型
大鼠
renal arteries
rats, inbred SHR
receptors, adrenergic, alpha
receptor subtypes
