摘要
目的：比较５～７ 天龄，５ ～６ 月龄以及１８ 月龄大鼠胸主动脉血管功能性α１肾上腺素受体（α１ａｄｒｅｎｏｃｅｐｔｏｒ，α１ＡＲ） 及其亚型分布。方法：采用离体血管收缩功能实验，利用不同的α１ＡＲ 亚型选择性拮抗剂，分别测定５ ～７天龄，５ ～６ 月龄以及１８ 月龄大鼠胸主动脉血管功能性α１ＡＲ 及其亚型。结果：由去甲肾上腺素（ＮＥ）引起的主动脉最大收缩反应在５～７ 天龄乳鼠显著低于５～６ 月龄及１８ 月龄大鼠。ＷＢ４１０１（α１ＡＡＲ和α１ＤＡＲ选择性拮抗剂）对ＮＥ介导的血管收缩效应的ｐＡ２ 值在５ ～７ 天龄，５～６ 月龄及１８ 月龄大鼠间差异无显著性，ＢＭＹ７３７８（α１ＤＡＲ选择性拮抗剂） 和Ｓｅｒｔｉｎｄｏｌ（α１ＡＡＲ 选择性拮抗剂） 在５～７ 天龄与５ ～６ 月龄大鼠的ｐＡ２ 值间差异也无显著性，但在１８ 月龄大鼠主动脉ＢＭＹ７３７８ 的ｐＡ２ 值显著减小；而Ｓｅｒｔｉｎｄｏｌｅ 的ｐＡ２ 值则明显增大。氯乙基可乐定（ＣＥＣ， 为α１ＢＡＲ和α１ＤＡＲ不可逆阻断剂） 对ＮＥ 引起的胸主动脉收缩的抑制作用在１８ 月龄大鼠明显小于５ ～６ 月龄大鼠。结论：在大鼠的生长过程中。
Objective: To compare functional α 1 adrenoceptor and its subtypes in isolated aortae among 5 7 day , 5 6 month and 18 month old rats. Methods: Vasoconstrictor response was measured. Results: The NE induced maximal contraction was significantly lower in 5 7 day old rats than that in the 5 6 month and 18 month old rats. The pA 2 value for WB4101 (an α 1A and α 1D selective antagonist) was not changed among 5 7 day , 5 6 month and 18 month old rats. The pA 2 value for BMY7378 (α 1D selective antagonist) and Sertindole (α 1A selective antagonist) had no difference between 5 7 day and 5 6 month old rats. The pA 2 value for BMY7317 was decreased, and that for Sertindole was increased in the 18 month old rats. The inhibitory effect of chlorethyclonidine (an irreversible antagonist for α 1B and α 1D AR subtypes) on NE induced contraction in 18 month old rats was weaker than that in 5 6 month old rats. Conclusion: During the development, α 1D subtype of α 1 adrenoceptor was mainly seen in both infancy and adult rat aortae. In aged rat aortae, the ratio of α 1A AR subtype of functional α 1 adrenoceptor was markedly increased.
出处
《北京医科大学学报》
CSCD
1999年第6期506-508,515,共4页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金
关键词
受体
肾上腺素能Α1
主动脉
年龄因素
Receptor subtype ☆ Receptors, adrenergic alpha 1/anal Aorta Age factors
