摘要
目的:通过检测DNA损伤标志物8-氧鸟嘌呤脱氧核苷(8-oxo-dG)在甲型H1N1型流感病毒感染MDCK细胞中的表达,初步探讨甲型H1N1型流感病毒诱导细胞凋亡的脱氧核糖核酸(DNA)损伤机制。方法:MDCK细胞培养后,甲型H1N1流感病毒感染继续培养0h、1h、3h、6h、12h、24h、48h,细胞爬片免疫组织化学方法检测各时间点DNA损伤标志物8-oxo-dG的表达。结果:甲型H1N1流感病毒感染后各时间点实验组MDCK细胞8-oxo-dG的表达均显著高于相应的正常对照组(P<0.05),病毒感染各组间两两比较发现各组间差异显著。结论:甲型H1N1流感病毒感染能造成宿主细胞DNA氧化损伤。
Objective：To explore the apoptosis mechanism of H1N1 influenza virus infection to MDCK cell by detecting the expression of 8-hydroxy-2＇-desoxyguanosine（8-oxo-dG） as biochemical marker of DNA damage.Methods：MDCK cells were seeded in slide of 48-well plate and infected with H1N1 influenza virus.Immunohistochemistry method was used to investigate 8-oxo-dG expression in MDCK cell respectively after being infected for 0,1,3,6,12,24 and 48 hours.Results：Significant difference of 8-oxo-dG in MDCK cells could be observed between every case-control pair at 0,1,3,6,12,24 and 48 hours;and significant increase of 8-oxo-dG could also be found among the experiment groups（P0.05）.Conclusion：H1N1 influenza virus infection can induce significant DNA oxidative damage.
出处
《中日友好医院学报》
2010年第5期289-291,F0004,共4页
Journal of China-Japan Friendship Hospital
基金
广东省自然科学基金博士科研启动项目(9451018201003654)
广东省中医药局建设中医药强省科研课题项目(2008422)
广州医学院博士启动基金项目(2008C17)
