摘要
目的:了解糖尿病患者h OGG1基因Ser326Cys位点多态性与冠脉病变之间的关系。方法:入选行冠状动脉造影术的糖尿病患者共323例,应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术检测每位患者的h OGG1基因Ser326Cys位点多态性。同时检测患者血糖、血脂、肾功能等生化指标。根据患者h OGG1基因Ser326Cys多态性分为3组:Cys/Cys基因型(85例)、Ser/Ser基因型(121例)和Ser/Cys基因型(117例)。由2名心血管医师对冠脉造影图像进行分析,按评判标准统计冠脉病变支数、病变复杂程度、Gensini评分和SYNTAX评分。分析h OGG1基因Ser326Cys多态性对h OGG1 mRNA表达和血8-OHd G水平的影响,以及对冠脉病变产生的影响。结果:(1)Cys/Cys基因型、Ser/Ser基因型和Ser/Cys基因型血8-OHd G水平差异显著(P<0.05)。组间两两比较,Cys/Cys基因型高于Ser/Ser基因型和Ser/Cys基因型,差异有统计学意义(P<0.05);而Ser/Ser基因型与Ser/Cys基因型比较,差异不显著(P>0.05)。(2)Cys/Cys基因型h OGG1 mRNA的表达低于Ser/Ser基因型和Ser/Cys基因型(P<0.05)。而Ser/Ser基因型与Ser/Cys基因型比较,h OGG1 mRNA表达差异不显著(P>0.05)。(3)Cys/Cys基因组出现3支病变的概率为37.6%,Ser/Cys基因型出现冠脉1支病变的概率为35.9%,但3组病变支数概率的差异均不显著(P>0.05)。(4)Cys/Cys基因型的Gensini和SYNTAX评分分别为48.7±15.3和39.5±17.2,冠脉复杂病变概率为73.0%,均高于Ser/Ser基因型和Ser/Cys基因型,差异显著(P<0.05)。而Ser/Ser基因型和Ser/Cys基因型之间比较,差异均无统计学意义(P>0.05)。结论:h OGG1基因Ser326Cys位点多态性与糖尿病患者冠脉病变有关,其中Cys/Cys基因型与病变严重程度有一定关系,其机制可能是Cys/Cys基因型的h OGG1表达水平下降,识别和切除DNA双链中的8-OHd G能力降低,修复DNA氧化损伤的能力下降,从而加速动脉粥样硬化的发生、发展。
AIM： To study the relationship between human 8-oxoguanine glycosylase 1（ h OGG1） gene Ser326 Cys polymorphism and severity of coronary artery lesions in the patients with diabetes mellitus. METHODS： We retrospectively analyzed 323 patients with diabetic mellitus receiving coronary angiography. These patients underwent the test of polymerase chain reaction-restriction fragment length polymorphism（ PCR-RFLP）,and were divided into Cys / Cys genotype（ n = 85）,Ser / Ser genotype（ n = 121） and Ser / Cys genotype（ n = 117） according to the results of PCR-RFLP.All clinical data including history of diseases,complications and biochemical markers,such as blood glucose,blood lipids and so on,were recorded. h OGG1 mRNA and 8-hydroxydeoxyguanosine（ 8-OHd G） were measured by RT-PCR and ELISA,respectively. The results of coronary angiography such as number and severity of coronary artery with lesions were analyzed by 2 cardiovascular physicians. Gensini score and SYNTAX score were also detected by the unitary criteria. RESULTS：（ 1） 8-OHd G in Cys / Cys genotype was higher than that in Ser / Ser genotype and Ser / Cys genotype（P〈0. 05）,but there was no statistical difference between Ser / Ser genotype and Ser / Cys genotype（P〈0. 05）.（ 2） h OGG1 mRNA expression in Cys / Cys genotype was lower than that in Ser / Ser genotype and Ser / Cys genotype（P〈0. 05）,and no statistical difference between Ser / Ser genotype and Ser / Cys genotype（P〈0. 05） was observed.（ 3） The probability of triple vessel lesions in Cys / Cys genotype was high and the probability of single vessel lesions in Ser / Cys genotype was low,but the difference among 3 genotypes was not statistically significant（P〈0. 05）.（ 4） Gensini score and SYNTAX score in Cys /Cys genotype were 48. 7 ± 15. 3 and 39. 5 ± 17. 2,respectively,and the ratio of complex lesions in Cys / Cys genotype was73. 0%. These scores and the ratio of complex lesions were higher than the other 2 genotypes（P〈0. 05）,an
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2015年第8期1477-1482,共6页
Chinese Journal of Pathophysiology
