摘要
目的利用ISSR分子标记技术鉴别阳春砂不同栽培品种,并分析其亲缘关系。方法利用ISSR-PCR方法对阳春砂栽培品种长果、圆果和"春选"与海南砂进行基因组多态性分析;采用NTSYS-pcversion2.10e软件计算各供试材料间的遗传相似系数,分析其亲缘关系并构建聚类树状图。结果 10条引物共扩增出114条DNA片段,其中49条DNA片段呈现多态性,占总扩增片段的43.0%。长果阳春砂与圆果阳春砂的遗传相似系数最大,高达1.000;长果、圆果阳春砂与海南砂的遗传相似系数最小,为0.577;各材料间的平均遗传相似系数为0.748。UPGMA聚类分析表明,长果阳春砂和圆果阳春砂聚成一支,再与"春选"阳春砂聚成一支,而海南砂独成一支。结论本研究结果鉴别了"春选"和阳春砂的另外两个栽培品种,证明了"春选"与海南砂有着较近的亲缘关系,建立基于ISSR分析的阳春砂遗传分析方法,为阳春砂的品种鉴别和优良品种选育提供了依据。
Objective To identify three cultivars of Amomum villosum Lour.and to analyze theirgenetic relationships by ISSR marker.Methods Genome polymorphism of three cultivars of Amomum villosum Lour.as well as Amomum longiligulare T.L.Wu was analyzed by ISSR-PCR.We calculated their genetic similarity coefficients by TSYS-pc version 2.10e,and analyzed their genetic relationship and constructed the clustering tree with UPGMA.Results A total number of 114 bands were amplified from 10 primers,among which 49 were polymorphic,accounting for 43.0 %.The genetic similarity coefficients were between 0.577 and 1.000,and the average coefficient was 0.748.The clustering tree showed that Changguo and Yuanguo were clustered into a subgroup firstly,and then they were clustered with Chunxuan into a group.Amomum longiligulare T.L.Wu formed a group alone.Conclusion Chunxuan and the othertwo cultivars of Amomum villosum Lour.have been identified in this study,indicating the closer genetic relationship between Chunxuan and Amomum longiligulare T.L.Wu.A genetic analysis method based on ISSR has been established,which provides evidence foridentification,selection and breeding of Amomum villosum Lour.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2010年第5期518-521,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家"十一五"科技支撑计划子项目(2006BAI09B01)
国家中药材扶持资金项目(粤经贸工业[2007]1055号)
广东省高等学校学科建设专项"中药学研究生创新培养基地"(粤教科函[2008]3号)
关键词
阳春砂
ISSR
分子鉴定
亲缘关系
Amomum villosum Lour.
ISSR
Molecularidentification
Genetic similarity
