摘要
利用微波辅助消解林蛙肝脏、骨粉、内皮和外皮组织样品,30min完成称样,消解到定容全部前处理过程,电感耦合等离子体光谱法1min检测1个样品中22种元素,省时、省力、快速、准确,准确度和精密度高于常规的湿法消化和低温干灰化法制备样品的方法,实验时选择微波消解时间15min,功率360W,混合酸体系为HNO3-HCl-H2O2-H2O,混酸配比为8∶2∶2∶3。微波消解林蛙皮肤样品大大缩短分析流程,采用标准溶液基体匹配法消除基体干扰,利用电感耦合等离子体原子光谱分析技术,同时检测林蛙组织中常量和微量元素。该方法经国家猪肝标准物质GBW08551验证,测得方法相对误差在0.17%～6.67%,相对标准偏差在0.47%～4.94%,各项检测指标均能满足生物样品检测要求,达到满意的测量结果。
By using the microwave-assisted method to digest the sample of forest frog’s liver,bone,endothelial and skin tissue,the research took 30 min to complete the whole pre-process including weighing,digesting and calibrating the volume of the sample.With the help of the Inductively coupled plasma spectrometry method,it took only 1 min to detect 22 elements in one sample.It is quick,convenient,accurate and preciser than the conventional wet digestion and dry ashing sample preparation method.This research used 15 min to digest the sample using microwave and the power was set to 360 W.The mixed acid system was HNO3-HCl-H2O2-H2O whose ratio was 8∶ 2∶ 2∶ 3.Using the microwave method to digest the sample of frog’s skin greatly shortened the period of analysis procedure.Choosing the standard solution medium matching method could eliminate the interference of the medium.At the same time,using the inductively coupled plasma atomic spectroscopy method,the constant and trace elements in the sample of forest frog tissue could be easily detected.This method was verified according to the national liver standard GBW08551.The relative error value of the result was tested between 0.17% ～ 6.67% and the relative standard deviation value was between 0.47% ～ 4.94% .Every detection index met the testing requirements of biological samples and the result was satisfied.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2010年第4期802-807,共6页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家自然科学基金资助项目(39970234)
