摘要
目的探讨中国林蛙卵油(eggoilofRanatemporariachensinensisDavid,EORTCD)抗惊厥作用及其与单胺神经递质的关系。方法以士的宁为致惊厥剂,评价EORTCD抗惊厥活性的时效和量效关系;采用离子对反相液相色谱-电化学检测法(HPLC-EC)测定小鼠大脑和脊髓内单胺类递质及其代谢产物,包括去甲肾上腺素(NA)、肾上腺素(E)、多巴胺(DA)和5.羟色胺(5-HT)、高香草酸(HVA),5-羟吲哚乙酸(5-HIAA)。结果灌胃给药EORTCD30min,其抗惊厥作用效果最好,呈现良好的剂量-效应关系;士的宁使脊髓的NA,5-HT,HVA显著减少,促进5-HT的消除,抑制多巴胺的转换。士的宁也抑制脑内多巴胺的转换。EORTCD可对抗士的宁对脊髓NA和HVA的抑制作用。恢复DA神经系统的代谢模式,对抗士的宁导致的5-HT下降和分解的加强,使5-HT的转换恢复到正常水平。对士的宁惊厥小鼠大脑单胺神经递质则无显著影响。结论推测EORTCD抗士的宁惊厥的途径是通过调节脊髓单胺神经递质而发挥作用。
OBJECTIVE: To investigate the anticonvulsion effect of Rana temporaria chensinensis David egg oil (EORTCD) and its relationship with monoamine neurotransmitters and their metabolites in spinal cord and brain of convulsion mice. METHODS: The anticonvulsion effects, dose-response and time-efficacy relationship were measured by using the model induced by strychnine. Monoamine neurotransmitters and their metabolites in CNS, including norepinephrine (NA), epinephrine (E), dopamine (DA), 5-hydroxytryptamine (5-HT), Homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA), were assayed by high performance liquid chromatography with electrochemical detector. RESULTS: The anticonvulsion effect was reached dose-dependently at 30 min after EORTCD was administered. Strychnine decreased 5-HT, NA and HAV in spinal cord, accelerated the clearance of 5-HT and inhibited the transition of DA in spinal cord. Strychnine also inhibited DA transition in brain. EORTCD opposed the decrease, restored DA metabolic model and recovered 5-HT level to normal. CONCLUSION: The inhibition action of EORTCD on CNS and the potent anticonvulsion effect on the model induced by strychnine are related to the monoamine neurotransmitters and their metabolites in spinal cord.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2005年第12期912-915,共4页
Chinese Pharmaceutical Journal
关键词
中国林蛙卵油
士的宁
抗惊厥
单胺神经递质
Brain
Detectors
Drug dosage
Liquid chromatography
Metabolism
Neurology
Organic compounds
Physiological models
