摘要
从角叉菜中提取卡拉胶，分级得到k-卡拉胶（KC），采用氯磺酸-吡啶法对其进行硫酸化修饰得到硫酸化卡拉胶（SKC），并测定了k-卡拉胶硫酸化前后硫酸基的含量：通过体外试验比较了KC及其硫酸化衍生物SKC对淋巴细胞增殖、巨噬细胞增殖活性以及NO释放活性的影响。结果表明，KC和SKC都能促进淋巴细胞和巨噬细胞的增殖活性，在样品实验浓度范围100～400mg／L内，SKC对T淋巴细胞和巨噬细胞的具有促增殖作用，增殖率分别为158．76％和153．63％，与KC相比具有显著性差异（P〈0．01）。SKC促进巨噬细胞产生NO的活性较KC更强，并呈一定的剂量的依赖性，SKC在400mg／L时，可促使巨噬细胞产生NO量为21．385μmol／mL。对KC进行硫酸化修饰，可以提高其对淋巴细胞巨噬细胞的免疫活性，能促进机体的免疫调节。
To study the effect of sulfating modification on the immumodulatory activities of k-carrageenan （KC）. The KC was sulfated using pyridine and chlorosulfonic acid, and then the sulfate content of the resulting sulfated KC （SKC） was determined. MTT colorimetric analysis was used to evaluate the improved proliferation of spleenlymphopoiesis and macrophage cell. NO production of murine peritioneal macrophages was detected using different in vitro model systems. The results indicated that SKC and KC improved proliferation of spleen lymphocyte and macrophage, SKC was more effective on the proliferation of spleen lymphocyte and macrophage than KC （p 〈 0.01）, At 100-400 mg/L, the best proliferation rate of spleen lymphocyte and macrophage were 158.76% and 153.63%, respectively. Additionally, SKC also increased more NO production in murine peritioneal macrophages than KC; at 400 mg/L, the production of NO was 21.385 μmol/mL. These results suggest that the SKC obtained by this derivatization can significantly improve the activity of spleen-lymphopoiesis and macrophage cell.
出处
《海洋科学》
CAS
CSCD
北大核心
2010年第8期56-59,共4页
Marine Sciences
基金
山东省优秀中青年科学家科研奖励基金项目(2008BS06012)
中国科学院海洋生物资源可持续利用重点实验室开放课题(LMB071005)
烟台市科技发展计划项目(2008GGA0020080410084458)
关键词
卡拉胶
硫酸化修饰
免疫活性
k-carrageenan
sulfated modification
immunomodulating effects
