摘要
目的优选党参多糖硫酸化工艺。方法用氯磺酸-吡啶法对党参多糖进行硫酸化修饰,以取代度和产物得率为指标,用L9(34)正交试验对试剂配比、反应温度、反应时间进行优选,用氯化钡-明胶法测定硫酸基的取代度。结果试剂配比和反应时间分别对产物得率和取代度的影响最大,氯磺酸与吡啶配比为1∶6时产物得率最高;反应时间为3 h时取代度最高,达1.83;各因素不同水平对产物得率和取代度的影响无显著差异。结论综合考虑最适宜的修饰条件为氯磺酸与吡啶配比为1∶6、反应温度80℃、反应时间3 h。
Objective To optimize the sulfated modification conditions of Codonopsis pilosula polysaccharides （CPPS）. Methods CPPS was modified by chlorosulfonic acid-pyridine L9（34）Orthogonal test was used to optimize reagent ratio,reaction temperature, and reaction time, taking substitution degree and productivity as index. Results The effects of reagent ratio and reaction time respectively on productivity and substitution degree were the strongest When the ratio of chlorosulfonic acid to pyridine was 1∶ 6, the productivity was the highest. When reaction time was 3 h, the substitution degree was the highest reaching 1.83. There were no significant differences among different levels of each factor. Conclusion The optimum modification conditions are the ratio of chlorosulfonic acid to pyridine of 1∶6, reaction temperature of 80℃, and reaction time of 3 h in synthetical consideration.
出处
《中草药》
CAS
CSCD
北大核心
2010年第7期1091-1094,共4页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(30871887)
国家科技支撑计划课题(2008BADB4B06)
关键词
党参多糖
硫酸化修饰
正交试验
Codonopsis pilosula polysaccharides （CPPS） sulfated modification orthogonal test
