摘要
目的筛选明党参多糖提取优化的最佳工艺。方法以明党参多糖为工艺筛选指标,采用正交实验设计方法筛选最佳提取工艺及其条件。结果明党参多糖最佳提取工艺为石油醚回流2h;滤渣用95%乙醇回流2h;滤渣再用10倍体积水回流浸提多糖。用sevage法除蛋白,加入乙醇使含醇量为80%,于冰箱静置12h,得多糖沉淀。采用优化后的工艺提取明党参多糖粗品的得率约为10%。结论采用该提取工艺,明党参多糖的提取率较高。
Objective To optimize the best extraction process of polysaccharides in root of Changium smyrnioides H. Wolff. Methods With polysaccharides of Changium as an examinable target component,the optimized extraction techniques and condition were chosen by orthogonal test method. Results The best extraction process of polysaccharides of Changium is that the crude drugs were extracted with petroleum ether for refluxed 2 h,the sediments extracted with 95% ethanol for 2 h first, then filtrable residue from previous steps were extracted with 10 times amount of water. The proteins were removed by Sevage＇s method, adding ethanol and making its concentration to 80%, the extracts were stored in the refrigeratory for 12 h. The acquired amount of Changium polysaccharides was about 10% from the best extraction process. Conclusion The extractive rate of Changium polysaccharides were higher in the optimized extraction process and the process was proved to be reasonable and feasible.
出处
《现代中药研究与实践》
CAS
2005年第4期51-53,共3页
Research and Practice on Chinese Medicines
基金
科技部十五攻关计划项目"水蛭和明党参规范化种植研究"(项目编号:2001BA701A6204)
关键词
明党参
多糖
提取工艺
Changium smyrnioides
polysaccharides
extraction process
