摘要
目的 :观察明党参多糖对转录活化核蛋白因子NF -κB(nuclearfactorofKappaB)结合活性的影响。方法 :在内毒素 (LPS)诱导J744 .a.1小鼠的巨噬细胞株 ,可诱生出较高的NF -κB活性 ,分别采用LPS先刺激、LPS后刺激和LPS与明党参多糖同时刺激等 3种方法对其处理 ,并分离核蛋白 ,行聚丙酰凝胶电泳及光密度扫描定量分析。结果 :发现明党参多糖在 3种情况下均可降低LPS刺激所致的NF -κB结合活性。结论 :明党参多糖可显著降低LPS刺激所致的NF
OBJECTIVE:To observe effection of Changan on transcription activating nuclear factor of kappa B(NF-κB).METHOD:The binding activity of NF-κB was increased in inacrophagocyte of J744.a.l mouse by LPS,nuclear protein were isolated and analyzed by electrophoresis on polyacrylamide gel,and quantified by scanning densitometry.RESULT:To discover that Changan reduced the binding activity of NF-κB.CONCLUSION:Changan can reduce the binding activity of NF-κB significantly.
出处
《南京中医药大学学报》
CAS
CSCD
1999年第6期356-357,共2页
Journal of Nanjing University of Traditional Chinese Medicine
基金
江苏省教委自然科学基金!项目 (940 60 )
