摘要
文章概述了几种除去多糖中蛋白质和色素的方法,以及应用有机溶剂沉淀法、季铵盐沉淀法、超滤法、离子交换色谱法、凝胶色谱法等分级纯化硫酸酯多糖的原理及方法,并从硫酸根含量、相对分子质量、总糖含量、纯度、结构等方面综述了分析硫酸酯多糖的方法,提出应对硫酸酯多糖的构效关系,并进行深入研究。
The thesis generalized several methods of removing proteins and eliminating pigment from polysac- charide, and several kinds of principles and methods that classify and purify sulfated polysaccharides, inclu- ding organic solvent precipitation, quaternary ammonium salt precipitation, ultrafiltration technology, ion ex- change chromatography and gel chromatography, then summarized the analytical methods of sulfated polysac- charides in terms of sulfate radical, relative molecular weight, total sugar, purity and structure. At last, the thesis put forward to further research structure -function relationship of sulfated polysaccharides.
出处
《福建水产》
2013年第2期155-160,共6页
Journal of Fujian Fisheries
基金
国家海洋公益专项(201205022-4)
关键词
硫酸酯多糖
分离纯化
纯度鉴定
结构分析
sulfated polysaccharides
isolation and purification
purity identification
structure analysis
