摘要
以新鲜扇贝闭壳肌为原料,酶法提取扇贝糖原,其得率为13.78%,其中糖质量分数为91.62%,蛋白质质量分数为1.4%。采用氯磺酸-吡啶法对所提取扇贝糖原进行硫酸酯化修饰,以硫酸取代度(DS)为指标,对硫酸酯化试剂体积、反应温度以及反应时间进行优化。实验结果显示,最佳修饰条件为酯化试剂中氯磺酸与吡啶的体积比为1∶5,反应温度为60℃,反应时间为3h。经红外光谱分析表明,硫酸基与扇贝糖原形成硫酸酯化合物。
The scallop muscle glycogen was obtained from scallop muscle by proteolytic extraction.The yield of glycogen was 13.78% and the contents of carbohydrate and protein were 91.62% and 1.4% respectively.The scallop muscle glycogen was modified by chlorosulfonic acid-pyridine(CSA-Pyr) method according to orthogonal test.The optimum modification conditions were the ratio of chlorosulfonic acid to pyridine of 1∶5,the reaction temperature of 60 ℃ and the reaction time of 3 h.FT-IR shows the formation of sulfated compounds.
出处
《大连工业大学学报》
CAS
北大核心
2012年第1期15-18,共4页
Journal of Dalian Polytechnic University
基金
国家高技术研究发展计划(863计划)项目(2011AA100803)
国家农转资金项目(2009GB2B000064)
辽宁省重点实验室项目(2008403003)
大连市科技攻关技术项目(2009B12NC014)
关键词
扇贝糖原
硫酸化修饰
硫酸取代度
scallop muscle glycogen
sulfating modification
degree of sulfation
