摘要
通过比较几种生长素和细胞分裂素物质对野葛（ｐｕｅｒａｒｉａｌｏｂａｔａ）外植体形态发生的影响发现，ＢＡ诱导的不定芽数量和质量均好于ＫＴ，最适浓度１ｍｇ·Ｌ１，同样浓度的ＢＡ比ＫＴ易使野葛外植体基部膨大和褐化，ＫＴ比ＢＡ更易使不定芽玻璃化，提高ＢＡ浓度或者不定芽在含ＢＡ或ＫＴ的培养基上继代，也会产生玻璃化和褐化，随继代次数增加而加重－在３种生长素类物质ＩＡＡ、ＩＢＡ和ＮＡＡ诱导不定根形成过程中，在０－２ｍｇ·Ｌ－１以下，ＮＡＡ诱导的效果最好，在０－３～０－４ｍｇ·Ｌ－１的浓度范围内，ＩＢＡ效果最好，而大于０－５ｍｇ·Ｌ－１的浓度则ＩＡＡ效果最好，在同样浓度条件下，ＮＡＡ诱导的不定根粗短，而ＩＢＡ和ＩＡＡ诱导的不定根细长，多分枝，形态上较接近自然根－３种生长素类物质诱导愈伤组织和褐化能力依次为ＮＡＡ＞ＩＢＡ＞ＩＡＡ，低浓度下生长素物质诱导的不定根生长快、生长质量好，幼苗移栽成活率高，而过高的生长素物质诱导的根系粗短，易褐化和形成愈伤组织、幼苗移栽不易成活，反映不定根与外植体维管束联系差－当ＢＡ与ＩＢＡ混合使用时，ＢＡ会严重抑制ＩＢＡ诱导的不定根形成，而ＩＢＡ对ＢＡ促进的不定芽分化效应作用不明显。
By comparing the effects of some auxin and cytokinin substances on organogensis of explants of Pueraria Lobato, it was found that the number and quality of adventitious buds induced by BA were better than those induced by KT, with the optimum concentration being 1 mg/L. The same concentration of BA caused swelling and browning of Pueraria Lobata explant basal more easily when compared to KT, but KT caused hyperhydriciting of adventitious buds more easily than BA. Increasing BA concentration or repeatedly subculturing adventitious buds in medium containing BA or KT also caused hyperhydriciting and browning. As far as the formation of adventitious roots induced by IAA, IBA and NAA was concerned, NAA yielded the best results below the concentration of 0.2 mg/L, IBA showed the best effects during 0.3～0.4 mg/L, while above 0.5 mg/L, IAA was the best. Under the same concentration, roots induced by NAA were thicker and shorter while roots induced by IAA and IBA were smaller and longer and had more branches. The capacity of inducing callus and browning followed the order of NAA>IBA>IAA. Roots induced by low concentrations of auxins grew well and had higher survival rate of plantlet transplanting. On the contrary, roots induced by high concentrations of auyins were thicker and shorter, browned and formed callus more easily, with a low survival rate of plantlet transplanting, indicating the poor interaction between buds and roots. When BA and IBA were mixed, BA seriously inhibited the formation of adventitious roots induced IBA, while no obvious effects of IBA on adventitious buds induced by BA were achieved. The optimum prescription for producing adventitious buds and roots at one step was BA 0.5 mg/L+IBA 0.3 mg/L.
出处
《华南师范大学学报（自然科学版）》
CAS
1999年第2期100-104,共5页
Journal of South China Normal University(Natural Science Edition)
基金
广东省重点科技攻关项目
