摘要
目的应用胰岛素(INSU)、阿仑膦酸钠(ALEN)干预体外高糖环境下培养的MC3T3-E1,探讨胰岛素、阿仑膦酸钠对MC3T3-E1增殖及凋亡的影响。方法用DMEM培养基培养MC3T3-E1细胞并以高糖(30 mmol.L-1)、INSU(10-6 mol.L-1)、ALEN(10-7 mol.L-1)进行干预,按①对照组(NG);②高糖组(HG);③高糖+胰岛素组(HG+INSU);④高糖+阿仑膦酸钠组(HG+ALEN);⑤高糖+胰岛素+阿仑膦酸钠组(HG+INSU+ALEN)进行分组,抽取24 h样本,采用对5-溴-2-脱氧尿嘧啶的嵌入法检测增殖率,应用流式细胞仪检测凋亡率。结果胰岛素和阿仑膦酸钠均可促进MC3T3-E1细胞增殖,HG+INSU+ALEN组促增殖作用最显著(P<0.05),在高糖环境下成骨细胞的凋亡率对于单一加用胰岛素和阿仑膦酸钠均有降低,与对照组相比有显著差异(P<0.05),但HG+INSU+ALEN组较其他组显著降低(P<0.01)。结论 INSU、ALEN可以增加MC3T3-E1细胞数量,使其凋亡率降低,INSU、ALEN对高糖环境下MC3T3-E1细胞具有保护作用。
Objective To investigate the effects of insulin （INSU） and alendronate （ALEN） on the proliferation and apoptosis of MC3T3-E1 osteoblasts in high concentration of glucose conditioned cultures. Methods MC3T3-E1 cells were cultured in the DMEM medium and additioned with high concentration of glucose （30 mmol · L-1 ） , INSU（ 10^-6 mol · L^-1 ） , and ALEN（ 10-7 mol · L^-1 ）. The cultures were divieded into five groups： （1） control group （NG）, （2） high concentration of glucose group （HG）, （3） high concentration of glucose and insulin group （HG ＋ INSU） , （4） high concentration of glucose and alendronate group （ HG ＋ ALEN） , and （5） high concentration of glucose, insulin, and alendronate group （ HG ＋ INSU ＋ ALEN）. Samples were extracted after 24 hours. Cell proliferation was measured using 5-bromo-2＇- deoxyuridine incorporation assay and the rate of apoptosis was measured using flow cytometry. Results Both INSU and ALEN promoted the proliferation of MC3T3-E1 cells and the most significant increase was found in HG ＋ INSU ＋ ALEN group （P 〈 0.05 ）. The apoptosis rate of osteoblasts in high concentration of glucose environment decreased after addition of INSU or ALEN comparing to NG （ P 〈 0. 05 ）. However, the apoptosis rate in HG ＋ INSU ＋ ALEN group significantly decreased comparing to other groups （ P 〈 0. 01 ）. Conclusion INSU and ALEN promote the proliferation of MC3T3-E1 osteoblasts, and reduce the apoptosis rate of these cells. INSU and ALEN have protective effects on MC3T3-E1 cells in high glucose environment.
出处
《中国骨质疏松杂志》
CAS
CSCD
2010年第8期553-557,共5页
Chinese Journal of Osteoporosis
