摘要
目的从细胞、蛋白及基因水平探讨辛伐他汀对成骨样细胞MC3T3-E1增殖的影响。方法辛伐他汀选取10-6、10-7、10-8和10-9mol/L4个浓度组,同时设溶剂对照组。含血清细胞培养条件下,连续细胞计数8d,描绘生长曲线;无血清培养条件下,分别于24、48及72h进行细胞计数、四唑盐比色试验(MTT)、蛋白质含量测定和DNA含量荧光测定。结果含血清细胞培养条件下,辛伐他汀各浓度组与溶剂对照组的生长曲线相比较,无明显差异;无血清培养条件下,辛伐他汀各浓度组与溶剂对照组24、48及72h时细胞计数、MTT比色试验及DNA含量荧光测定各组间未见明显差异;蛋白质含量测定24及72h时各组间无明显差异,然而48h时10-6mol/L辛伐他汀组蛋白含量明显高于对照组并差异有显著性(P<0.05)。结论辛伐他汀促进了成骨样细胞MC3T3-E1细胞的蛋白合成功能,但对其增殖无明显影响。
Objective To study the effect of simavastatin on the osteoblast-MC3T3-E1 proliferation from the levels of cell, protein and gene. Method Five test groups included simavastatin groups (10^-6 , 10^-7 , 10^-8 , 10^-9 mol/L) and vehil control group. Under cell culcure with serum, cell growth curves for 8 days was drawn; Under cell culcure without serum, cell count, MTT test, DNA content and protein quantity within 24 hs,48 hs and 72 hs were tested. Result There are no significant differences between simvastin groups and control group in growth curves, cell count, MTT test and DNA content. Protein content of 10^-6M simvastatin group was significantly higher in the 48th hours of cell culture without serum, compared with control group( P 〈 0.05). Conclusion Simvastatin increased the protein content of MC3T3-E1, but didn't affect the cell proliferation.
出处
《中国骨质疏松杂志》
CAS
CSCD
2008年第2期101-105,共5页
Chinese Journal of Osteoporosis