摘要
目的探讨体外注射重组人促红细胞生成素(recombinant human erythropoietin,RhEPO)对新生鼠缺氧缺血后脑组织一氧化氮(nitric oxide,NO)水平及脑细胞凋亡的影响。方法 7日龄Wistar大鼠101只,随机分成3组,假手术组(n=25)、对照组(生理盐水组)(n=38)、实验组(RhEPO组)(n=38)。对照组与实验组建立HBID模型,模型制备后即刻实验组腹腔注射RhEPO 4000 U/kg,对照组注射同体积生理盐水,假手术组不结扎颈总动脉,不缺氧。各组于处置后不同时间段处死取脑组织,制作脑组织匀浆,测定NO水平。同时于术后24hTunel染色,观察海马区神经细胞凋亡情况。结果对照组与假手术组比较NO2h显著升高(P<0.05),12-96h差异有显著性意义(P<0.01)。实验组与对照组比较,NO明显降低,24-96h差异有显著性意义(P<0.01)。Tunel染色对照组海马区凋亡细胞数明显高于实验组。结论 RhEPO抑制新生鼠缺氧缺血脑组织NO过量产生及神经细胞凋亡,对HIBD起保护作用。
Objective：To explore the influence of recombinant human erythropoietin（RhEPO） on NO and neuronal apoptosis in the brain tissue of hypoxic-ischemic brain damage（HIBD） in neonatal rats.Methods： 101 postnatal seven-days-old Wister rats were divided into three groups,Sham operation group（n= 25）,control（NOrmal saline NS） group（n= 38）,experiment（RhEPO）group（n= 38）.Control group and experimental group were established HBID models,The rats of experimental group were injected intraperitoneally into RhEPO（4000 U/ kg）,control group was injected into equal volume NS after HIBD.The rats of Sham operation group were dissociated of carotid artery without ligation.Rats were killed at different time after the treatment and NO level of brain tissue was measured.Tunel staining was used to detect apoptosis in the hippocampal region.Results ：Control group compared with Sham operation group,NO markedly increased at the 2 hours（P0.05）,in the 12-96 hours（P0.01）.By Tunel staining,the apoptosis cells in the hippocampal region of the observation group decreased compared with the control group.Conclusion ：RhEPO has cerebral protective effects that gets by inhibiting NO excessive production and neuronal apoptosis after HIBD.
出处
《泰山医学院学报》
CAS
2010年第5期334-336,共3页
Journal of Taishan Medical College
关键词
重组人促红细胞生成素
脑缺氧
脑缺血
一氧化氮
凋亡
recombinant human erythropoietin
cerebral hypoxic
cerebral ischemic
NO
apoptosis
