摘要
为探明冷应激对雏鸡下丘脑NO代谢的影响,以公雏鸡为实验动物,进行急性(1 h、6h及12 h)与慢性(5 d1、0 d及20 d)冷应激处理(12℃±1℃),检测了下丘脑NO含量i、NOS与T-NOS活性以及iNOS基因mRNA的表达水平。结果,急性应激时,NO含量i、NOS与T-NOS活性以及iNOS基因mRNA的表达水平均随应激时间的延长逐渐降低;慢性应激时,NO含量i、NOS与T-NOS活性以及iNOS基因mRNA的表达水平均随应激时间的延长逐渐升高。结果表明,冷应激可导致下丘脑NO的代谢发生改变,不同程度的冷应激对NO代谢产生的影响也不同。
To explore the effect of cold stress on hypothalamic NO metabolism in chickens,male ISA chickens were subjected to acute（for 1 h,6 h and 12 h,respectively） and chronic（for 5 d, 10 d and 20 d,respectively） cold stress （12 ℃±1 ℃）. NO contents,iNOS and T-NOS activities,and iNOS mRNA levels in hypothalamus of the chicken were examined. The results were as follows. NO contents,iNOS and T-NOS activities, and iNOS mRNA levels all gradually decreased with the time lapsing during acute cold stress. NO contents,iNOS and T-NOS activities,and iNOS mRNA levels all gradually increased with the time lapsing during chronic cold stress. The results suggested that cold stress could play a role in regulation and toxicity of the organism through the alteration of NO metabolism, and the different extent cold stress would produce different effects on NO metabolism.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2007年第5期429-434,共6页
Chinese Veterinary Science
