摘要
目的观察中缝背核(DRN)内一氧化氮合酶(NOS)对炎性痛大鼠脊髓伤害性信息传递的调控作用。方法建立大鼠单侧足底甲醛炎性痛模型,采用行为学、c-Fos免疫组织化学及烟酰胺腺嘌呤二核苷酸磷酸黄递酶(NADPH-d)组织化学技术,观察甲醛诱发炎性痛后大鼠DRN内NADPH-d、NADPH-d/Fos双标阳性神经元数量的变化;同时观察DRN内预先给予NOS抑制剂L-NAME对甲醛致痛大鼠疼痛评分及脊髓Fos蛋白表达的影响。结果单侧足底注射甲醛后,DRN内NADPH-d、NADPH-d/Fos双标阳性神经元的数量增加。DRN内预先注射L-NAME降低炎性痛大鼠的疼痛学评分及脊髓Fos阳性神经元的数量。结论 DRN内NOS可能促进甲醛炎性痛大鼠脊髓伤害性信息的传递。
Objective To investigate the role of nitric oxide nitric oxide synthase（NOS） in modulating inflammatory nociceptive transmission in dorsal raphe nuclei（DRN） of spinal cord in rats.Methods The changes in the numbers of nicotinamide adenine dinucleotide phosphate-diaphorase（NADPH-d） and Fos/NADPH-d double labeled positive neurons in the DRN were observed with formaldehyde test,Fos immunocytochemistry and histochemistry methods on rat experimental pain model.The effects of formaldehyde（L-NAME） preinjection into the DRN on the paw pain score and Fos expression were recorded as well.Results The numbers of NADPH-d and Fos/NADPH-d double labeled positive neurons in the DRN and the paw pain score and Fos expression were increased,which was decresed by L-NAME.Conclusion NOS located in the DRN possibly facilitates formaldehyde-induced spinal cord nociceptive transmission.
出处
《江苏医药》
CAS
CSCD
北大核心
2010年第15期1793-1795,F0003,共4页
Jiangsu Medical Journal
基金
江苏省卫生厅重点实验室开放课题(KJS06003)
关键词
中缝背核
一氧化氮合酶
炎性痛
Dorsal raphe nuclei
Nitrc oxide synthase
Inflammatory pain
