摘要
目的:探讨纳米细菌(nanobacteria,NB)在结石患者血清的感染情况,并对其进行形态学观察和特征描述。方法:采集北京大学人民医院泌尿外科24例行经皮肾镜碎石术治疗的肾结石患者和3例健康志愿者的血清进行NB的培养,应用免疫组织化学、von kossa染色、扫描电镜、透射电镜等方法对培养的NB予以观察和鉴定。结果:经鉴定,24位结石患者中有22例感染NB,而健康志愿者组未见NB感染,结石患者组NB的感染率高于健康对照组。培养4周后,NB在光镜下成球形,直径约100～500nm,能够被免疫组织化学和von kossa染色特异的识别。NB在透射电镜(负染法)下具有"毛刺"状磷灰石外壳、内部为着色较浅的菌体结构,并能够进行出芽繁殖。结论:绝大多数结石患者的血清中可以培养出NB,且阳性率高于健康对照组,这说明它可能与肾结石形成密切相关。免疫组织化学、von kossa染色、扫描电镜、透射电镜都是在不同方面对NB进行检测和鉴定的特异方法。NB的特异形态与特征是它区别于其他纳米颗粒的重要依据。
Objective：To detect,culture,and characterize the nanobacteria（NB） from sera of patients with kidney calculi in our department.Methods：Blood samples of 24 patients with kidney calculi and of 3 healthy volunteers in our department were collected for NB culture in this study.We used immunohistochemistry,von kossa staining,scanning electron microscopy（SEM）,transmission electron microscopy（TEM） to investigate the appearance and components of cultural NB.Results：Twenty-two blood samples out of 24（91.67%） showed growth of NB,while no NB were detected in volunteers＇ blood samples.The infection rate of stone group was obviously higher than that of healthy volunteers.After a 4-week culture period,the light microscope revealed coccoid-shaped NB with a diameter of 100-500 nm,which could be identified by immunohistochemistry and von kossa staining.SEM and TEM（negative staining） revealed NB with a hollow interior coated in needle-like apatite crystals.Such nanopaticles could bud-off new ones and therefore appeared like living organisms.Conclusion：NB can be identified from sera of most patients involved in kidney calculi.It may have intimate relation to the formation of kidney calculi because the infection rate of NB blood samples of stone patients was significantly higher than that of healthy volunteers.Immunohistochemistry,von kossa staining,SEM and TEM are special methods for identifying NB from different aspects.The appearance and character are important points to distinguish NB from other nano-sized particles.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2010年第4期443-446,共4页
Journal of Peking University:Health Sciences
基金
国家自然科学基金(40772026)资助~~
关键词
肾结石
纳米细菌
感染
血清
Kidney calculi
Nanobacteria
Infection
Serum
