摘要
目的：研究重组人纤连蛋白ＣＨ５０对小鼠黑色素瘤生长的影响及其抗肿瘤机制．方法：进行小鼠动物实验及黑色素瘤Ｂ１６细胞实验．结果：ＣＨ５０在体内明显抑制黑色素瘤生长及实验性肺转移．ＣＨ５０在体外能粘附黑色素瘤Ｂ１６细胞，抑制Ｂ１６细胞粘附层粘蛋白并明显提高腹腔巨噬细胞对Ｂ１６细胞的杀伤活性．结论：ＣＨ５０抑制小鼠黑色素瘤的生长和转移，ＣＨ５０的抗肿瘤机制与其粘附肿瘤细胞，提高巨噬细胞杀瘤活性有关．
AIM: To study the effect of recombinant human fibronectin polypeptide CH50 on murine melanoma growth and metastasis, and its antitumor mechanism. METHODS: Mouse and melanoma B16 cell tests were used to observe antitumor effect and mechanism of CH50. RESULTS: CH50 markedly inhibited melanoma growth and experimental lung metastasis. The melanoma weight was reduced from (2 3±1 2) g of control group to (0 7±0 8) g of test group ( P <0 05). CH50 at 0 125, 0 25, 0 5 mg/mouse reduced melanoma lung metastatic colonies from 87±49 of control group to 34±6, 14±12, 4±2, respectively. CH50 adhered to melanoma B16 cells and inhibited adhesion of B16 cells to laminin. CH50 enhanced the cytotoxicity of macrophages to melanoma B16 cells. CONCLUSION: CH50 inhibited tumor growth and metastasis of murine melanoma. The antitumor effect of CH50 is related to its adhesion ability to melanoma B16 cells and enhancing macrophage cytotoxicity.
出处
《中国药理学报》
CSCD
1999年第2期175-178,共4页
Acta Pharmacologica Sinica
关键词
重组蛋白
纤连蛋白
CH50
黑色素瘤
抗肿瘤药
recombinant proteins
fibro￣nectins
laminin
peptides
experimental mela￣noma
cultured tumor cells
antineoplastic agents
