摘要
目的通过检测Foxp3在黑色素瘤B16细胞中的表达及其对肿瘤细胞增殖的影响,探讨Foxp3在黑色素瘤细胞中表达的可能作用.方法采用RT-PCR和免疫组化法检测B16细胞中Foxp3在mRNA和蛋白水平的表达;采用VigoFect转染试剂将pcDNA3.1-Foxp3真核表达载体瞬时转染B16细胞,RT-PCR和流式细胞术方法分别检测转染前后Foxp3基因和蛋白的表达;MTT方法检测Foxp3高表达后对肿瘤细胞增殖的影响.结果 B16细胞在mRNA和蛋白水平均表达Foxp3;瞬时转染后Foxp3转染组中Foxp3的表达显著高于空载体组和B16组(P<0.01);Foxp3转染组细胞A490 nm值在48 h和72 h与空载体组和B16组相比显著降低,差异具有统计学意义(P<0.05).结论黑色素瘤B16细胞Foxp3的表达可抑制肿瘤细胞的增殖.
Objective To explore effects of Foxp3 expression on the proliferation in melanoma B16 cells by detecting the expression of Foxp3 in melanoma B16 cells and its effects on the cell proliferation. Method Foxp3 mRNA was detected by RT-PCR and Foxp3 protein was detected by immunocytochemical staining. The plasmid pcDNA3. 1-Foxp3 was transfected into B16 cells by VigoFect. Expressions of Foxp3 gene and protein before and after the transfection in B16 cells were detected by RT-PCR and flow cytometry,respectively. The effect of overexpressed Foxp3 on the proliferation of B16 cells was detected by MTT. Results Foxp3 mRNA and protein could be detected in B16 cells. The expression of Foxp3 was significantly higher in Foxp3 transferred cells than that of empty plasmid transferred cells and B16 cells（ P〈0. 01）. The transferred cells A490 in Foxp3 group were much lower than those in empty plasmid group and B16 group at 48 h and 72 h.（ P〈0. 05）. Conclusion The expression of Foxp3 could inhibit the cell proliferation in B16 cells.
出处
《北华大学学报（自然科学版）》
CAS
2014年第5期606-609,共4页
Journal of Beihua University（Natural Science）
基金
吉林市科技局基金项目(201262501)
