摘要
在pH6.0的HAc-NaAc缓冲溶液中,血红蛋白与水溶性的CdTe量子点作用生成聚集体,引起体系共振瑞利散射的显著增强,其最大散射峰位于460 nm.研究pH值、量子点浓度、离子强度等条件对体系共振瑞利散射光强度的影响.结果表明,共振散射光强度与血红蛋白的浓度在6.6-189.2 mg/L范围内呈良好的线性关系,线性回归方程为△IRRS=139.9＋1.123C,相关系数为0.992 8,检出限为0.5 mg/L.用于实际样品测定,回收率为97.0%~103.4%范围内,相对标准偏差为3.2%~6.1%.据此建立了一种快速、灵敏测定血红蛋白的新方法.
In HAc-NaAc buffer（pH 6.0）,hemoglobin reacted with CdTe quantum dots to form ionassociation complexes,which greatly enhanced the resonance Rayleigh scattering（RRS） intensity of the system at the wavelength of 460 nm.The optimum reaction conditions including pH,the concentration of CdTe quantum dots and the intensity of ionic of the system were investigated.Under optimum condition,the RRS intensity（ΔI） at 460 nm was linear to the concentration of hemoglobin in the range of 6.6-189.2 mg/L.The regression equation was △IRRS = 139.9 ＋ 1.123 C,with a correlation coefficient of 0.992 8.The detection limit was 0.5 mg/L.Based on this,a novel method for the determination of trace amounts of hemoglobin by using the RRS method was developed and used for the analysis of human serum samples.The recoveries were in the range of 97.0%-103.4%,and the RSDs were 3.2%-6.1%.The proposed method was simple,sensitive and rapid.
出处
《信阳师范学院学报（自然科学版）》
CAS
2010年第3期443-446,共4页
Journal of Xinyang Normal University(Natural Science Edition)
基金
河南省科技攻关项目(0524440059)
河南省高校科技创新人才基金项目(2010HASTIT025)
河南省科技创新杰出青年基金项目(104100510020)
