摘要
糖尿病是世界上发病率最高的疾病之一。目前,检测糖化血红蛋白已经成为糖尿病监控的标准诊断方法。血红蛋白在人体中承担着重要作用,是主要担负着运载氧的功能的大分子蛋白质,糖尿病患者过高的血糖会诱使葡萄糖和血红蛋白结合,生成没有功能的糖化血红蛋白,影响血液的输氧能力,因此血液中的糖化血红蛋白的检测意义重大。临床实验室常用的测定糖化血红蛋白的方法有30多种,根据反应原理的不同可分为两大类:第一类基于GHb与非GHb的电荷不同,包括离子交换色谱法、电泳法、等电聚焦法等;第二类基于GHb的结构特点,包括亲和色谱法、离子捕获法、免疫法等。本文主要对临床常用的糖化血红蛋白检测方法进行了综述。
Diabetes mellitus is one of the highest morbidity in the world. At present, the detection of glyco-sylated hemoglobin has become the standard diagnostic method of diabetes monitoring. Hemo-globin plays an important role in human body, as a macromolecular protein which is mainly re-sponsible for carrying oxygen. And the combination of high blood glucose in patients with diabetes will induce glucose and hemoglobin in HbA1c generation function, blood oxygen capacity, so the determination of glycosylated hemoglobin in blood shows great significance. There are more than 30 methods used for the determination of glycosylated hemoglobin in clinical laboratories. According to the principle of the reaction, which can be divided into two categories, the first category is based on the different charge of GHb and non GHb, including ion exchange chromatography, electrophoresis, isoelectric focusing, etc. The second category is based on the structural characteristics of GHb, including affinity chromatography, ion capture, immunization, etc. This article overviewed detection methods of glycosylated hemoglobin in clinical practice.
出处
《分析化学进展》
2017年第3期163-170,共8页
Advances in Analytical Chemistry
基金
大学生创新创业计划项目(201510595044,201510595207,201510595057)资助
国家自然科学基金项目(61301038,61661014)资助。
