摘要
目的评价利奈唑胺(噁唑烷酮类抗菌药)、万古霉素(胺基糖苷类抗生素)2种抗菌药物对耐甲氧西林金黄色葡萄球菌(MRSA)的体外抗菌活性。方法用琼脂二倍稀释法,测定抗菌药物的最低抑菌浓度(MIC);用肉汤稀释法,测定抗菌药物的最低杀菌浓度(MBC),绘制杀菌曲线(KCs)。结果利奈唑胺对临床分离的98株MRSA的MIC50为1μg.mL-1,MIC90为2μg.mL-1,MBC50为8μg.mL-1,MBC90为32μg.mL-1,敏感率为100%;万古霉素对临床分离的98株MRSA的MIC50为1μg.mL-1,MIC90为1μg.mL-1,MBC50为8μg.mL-1,MBC90为32μg.mL-1,敏感率为100%。随着抗菌药物浓度的升高,其杀菌时间缩短不甚明显,呈现非浓度依赖性的特点。结论利奈唑胺对MRSA的体外抗菌活性与万古霉素相当,均显示非浓度依赖性的杀菌曲线。
Objective To evaluated the in vitro antimicrobial activities of linezolid and vancomycin agents against methicillin -resistant Staphylococcus aureus （MRSA）. Methods The minimal inhibitory concentrations （MICs） of antibacterial agents were determined by the standard two -fold agar dilution method. The minimal bactericidal concentrations （MBCs） antibacterial agents were determined by tube broth dilution method and time -killing curves were obtained. Results The sensitive rates to linezolid and vancomycin against MRSA were both 100%, the MIC50 and MIC90 were 1 μg·mL^-1,1 μg·mL^-1 and 2 μg·mL^-1, 1 μg·mL^-1. 32 μg·mL^-1 respectively. The MBC50 and MBC90 were both 8μg·mL^-1,32μg·mL^-1, respectively. With the increase of drug concentrations, the killing time did not show obvious decline. Conclusion Linezolid and vancomycin have strong antibacterial activity against MRSA, and they have nonconcentration independent characteristic.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2010年第8期593-596,共4页
The Chinese Journal of Clinical Pharmacology
基金
国家自然科学基金资助项目(30873127)
关键词
利奈唑胺
万古霉素
耐甲氧西林金黄色葡萄球菌
最低抑菌浓度
杀菌曲线
linezolid
vancomycin
methicillin - resistant Staphylococcus Staphylococcus
minimal inhibitory concentration
timekilling curve
