摘要
目的观察亚最低杀菌浓度(Sub-minimum bactericidal concentration,Sub-MBC)阿莫西林对金黄色葡萄球菌标准菌株的耐药性影响。方法以金黄色葡萄球菌标准菌株(ATCC 29213)为研究对象,阿莫西林为抗菌药物,以微量稀释法定量检测不同诱导天数MBC值,采用1/2MBC浓度对金黄色葡萄球菌进行30 d多步体外诱导实验,观察金黄色葡萄球菌标准菌株MBC变化,以全自动微生物药敏鉴定仪对最终诱导后菌株进行阿莫西林耐药性分析。结果金黄色葡萄球菌标准菌株MBC值阿莫西林体外诱导7 d即显著升高,诱导30 d升高至原始菌株MBC的32倍;经全自动药敏分析仪鉴定诱导出的耐药菌株对阿莫西林耐药。结论亚最低杀菌浓度阿莫西林可体外诱导金黄色葡萄球菌标准菌株出现耐药,且耐药性随亚最低杀菌浓度诱导时间增加而升高,提示合理选择抗菌药物使用剂量可作为预防细菌耐药重要措施。
Objective To observe the effects of inducing by amoxicillin with the dose of sub-MBC（ minimum bactericidal concentration） on the antibiotic resistance of Standard strains of staphylococcus aureus. Methods The standard strain ATCC 29213 of staphylococcus aureus were induced by amoxicillin with the dose of 1 /2MBC for 30 days. The quantitative data of MBC were detected by microdilution method in vitro everyday,and the antibiotic resistance to amoxicillin of staphylococcus aureus induced by this method were identified using automatic microorganisms drug sensitivity analyzer. Results The quantitative data of MBC of the standard strain of staphylococcus aureus induced by amoxicillin for 7 days significantly increased,and the MBC increased 32 times after induced for 30 day than that of the original strain. The drug-resistant strain induced by our methods showed resistant of amoxicillin which was identified by the automatic microorganisms drug sensitivity analyzer. Conclusion Amoxicillin can induce the standard strains of staphylococcus aureus resistance to antibiotic with the dose of sub-MBC for long time in vitro,and the degree of the antibiotic resistance increased by the time of inducement with sub-MBC,suggesting that it is important to to select a rational dosage of antimicrobial to prevent antibiotic resistance in clinics.
出处
《东南国防医药》
2015年第5期458-460,495,共4页
Military Medical Journal of Southeast China
基金
江西省卫生厅资助课题(20092108)
