摘要
采用核磁共振波谱分析技术,确定桔子原汁中葡萄糖、果糖、蔗糖及柠檬酸在重水中的各种构型,对桔子原汁中葡萄糖、果糖、蔗糖及柠檬酸进行指纹归属。运用模拟谱证实了桔子原汁中存在葡萄糖、果糖、蔗糖和柠檬酸。以邻苯二甲酸氢钾作内标,利用质子核磁共振谱的主要特征峰同时对桔子原汁中葡萄糖、果糖、蔗糖及柠檬酸进行定量分析。葡萄糖、果糖、蔗糖和柠檬酸测定结果的RSD(n=6)分别为3.61%、3.24%、2.19%、4.6%;葡萄糖、果糖、蔗糖和柠檬酸的回收率分别为99.4%、98.8%、97.8%、95.7%。该方法取样量少、简单、快速、准确,可以同时测定桔子原汁中葡萄糖、果糖、蔗糖和柠檬酸含量。
Various configurations of glucose,fructose,sucrose and citric acid in raw orange juice were determined by NMR when dissolved in heavy water.Glucose,fructose,sucrose and citric acid were attributed by fingerprint. Simulated spectra confirmed glucose,fructose and sucrose citric acid’s existence in raw orange juice.Potussium biphthalate was selected as an internal standard,1H-NMR spectra was used to determine the content of glucose, fructose and sucrose citric acid at the same time.RSD（ n = 6） of glucose,fructose and sucrose citric acid were 3.61%,3.24%,2.19% and 4.6% respectively,recovery of them were 99.4%,98.8%,97.8% and 95.7%,respectively. This method used only a little sample and was simple,rapid,accurate and suitable for the determination of the contents of glucose,fructose,sucrose and citric acid in raw orange juice at the same time.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第8期353-357,共5页
Science and Technology of Food Industry
