摘要
本文利用纤维素酶对花生壳进行酶解,以溶液pH、酶解温度、酶解时间和酶的添加量为试验因素,以酶解率为考察指标,采用单因素试验和正交试验对花生壳的酶解工艺条件进行了研究。结果表明,花生壳的最佳酶解条件为pH=5.5、酶解温度为45℃、酶解时间为18h、酶的添加量为1.5%,在此条件下进行试验,测得酶解率为63.61%。
Enzymatic hydrolysis technology of peanut hulls by cellulase was investigated by single-factor test and orthogonal test according to the degree of hydrolysis.Enzymolysis temperature,enzymolysis time,enzymolysis pH and cellulase addtition were selected as the experimental factors.The result showed that the optimum enzymolysis conditions were pH= 5.5,enzymolysis temperature of 45℃,enzymolysis time of 18h and cellulase addition of 1.5%.Under above conditions,the enzymolysis rate is 63.61%.
出处
《青岛农业大学学报（自然科学版）》
2012年第3期218-222,共5页
Journal of Qingdao Agricultural University(Natural Science)
基金
山东省自然科学基金项目(ZR2010CL027)
山东省高等学校优秀青年教师国内访问学者项目(2007-11-2010D104)
关键词
花生壳
纤维素酶
酶解
工艺条件
peanut hull
cellulase
enzymolysis
technological conditions
