摘要
以大西洋马铃薯(cv.Atlantic)块茎为试材,研究了壳聚糖处理及干腐病菌Fusarium sulphureum挑战接种对马铃薯块茎组织活性氧(ROS)代谢的影响,结果表明:壳聚糖处理能显著提高马铃薯切片过氧化氢(H2O2)、超氧阴离子(O2-)和抗坏血酸(AsA)含量及过氧化氢酶(CAT)和谷胱甘肽还原酶(GR)活性,而降低超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)活性,F.sulphureum挑战接种可进一步提高壳聚糖处理及对照马铃薯切片的H2O2和O2-的含量及CAT、APX和GR的活性。可见壳聚糖和病原物可通过诱导氧迸发而启动马铃薯组织的抗病反应。
Effect of chitosan treatment and Fusarium sulphureum change inoculation on reactive oxygen species metabolism in potato tuber slices was studied.The results showed that chitosan significantly increased the contents of hydrogen peroxide（ H2O2） ,super oxide radical（ O2） and ascorbic acid（ AsA） and activities of catalase（ CAT） and glutathion reductase（ GR） ,however activities of superoxide dismutase（ SOD） and ascorbic peroxidase（ APX） were decreased.The activities of CAT,APX and GR and the production of H2O2 and O2-were enhanced furthermore by inoculated with F.sulphureum in the treated tissue and the control.It was proposed that chitosan as a chemical activator could induce oxidative burst and then initiate disease defence response of potato tuber tissue.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第8期313-315,328,共4页
Science and Technology of Food Industry
基金
农业部农业公益性行业科研专项经费项目(NYHYZX07-6)
甘肃省农业生物技术项目(GNSW-2005-08)
关键词
壳聚糖
活性氧代谢
马铃薯块茎
硫色镰刀菌
干腐病
chitosan
reactive oxygen species metabolism
potato tuber
Fusarium sulphureum
dry rot
