摘要
目的建立中药连翘中连翘苷和连翘酯苷的HPLC含量测定方法。方法采用MerckC18柱(250mm×4.6mm,5μm),连翘苷测定流动相为乙腈-水(25:75),检测波长为277nm;连翘酯苷测定流动相为甲醇(含1%四氢呋喃)-0.01mol·L-1磷酸二氢钾水溶液(用磷酸调pH至3.2)梯度洗脱,检测波长为332nm。结果理论板数以连翘苷和连翘酯苷计算均不低于5000,两物质色谱峰与相邻峰之间分离度大于1.5,对称因子均在0.95～1.05之间。连翘苷和连翘酯苷的线性范围分别为50.4～252.2mol·L-1和20.5～102.5mol·L-1,平均回收率为100.3%和99.2%,RSD分别为1.52%和1.86%。结论本实验建立的色谱方法灵敏、准确、重现性好,可用于中药材连翘的质量控制。
【Objective】To establish a method for determination of phillyrin and forsythoside A in Forsythia suspensa.【Methods】A Merck ODS-C18 column（250 mm × 4.6 mm,5 μm） was used.For determination of phillyrin,the mobile phase was acetonitrile-water（25：75）,and deterting wavelength was 277 nm.For determination of forsythoside A,the mobile phase was methanol（containing 1% THF）-0.01 mol· L-1 potassium dihydrogen phosphate solution（pH 3.2） as gradient elution,and deterting wavelength was 332 nm.【Results】The calibration curve was linear in the range of 50.4 ～ 252.2 mg· L-1（r = 0.9999） for phillyrin and 20.5 ～ 102.5 mg· L-1（r = 0.9999） for forsythoside A.The average recovery in sample was 101.3% and 99.2% for phillyrin（RSD 1.58%） and forsythoside A（RSD 1.86%）,respectively.【Conclusions】The method is sensitive,accurate and simple,and it＇s suitable for the quality control of Forsythia suspensa.
出处
《中国医学工程》
2010年第2期46-48,52,共4页
China Medical Engineering
