摘要
目的:检测鼻咽癌组织中核因子-kappa B(NF-κB)/p65、基质金属蛋白酶(MMP)-3、MMP-9的表达,探讨鼻咽癌组织中NF-κB/p65、MMP-3、MMP-9的表达与临床意义,以及三者之间的相互关系。方法:运用免疫组织化学方法(SP法)检测鼻咽癌和正常鼻咽部黏膜的活检标本中NF-κB/p65、MMP-3、MMP-9的表达情况,运用χ2检验和Spearman等级相关检验等方法分析其表达与病理分型等临床资料的关系,以及三者之间的相互关系。结果:NF-κB/p65在鼻咽癌中的表达率为78.0%,MMP-3、MMP-9在鼻咽癌中的表达率都为75.6%,与在正常鼻咽部黏膜中的表达差异有统计学意义(P<0.05)。NF-κB/p65、MMP-3、MMP-9在鼻咽癌中的表达与淋巴结转移有显著相关性(P<0.05)。NF-κB/p65在鼻咽癌中的表达与MMP-3、MMP-9呈正相关,MMP-3和MMP-9之间亦有正相关。结论:NF-κB/p65、MMP-3、MMP-9在鼻咽癌组织中有广泛高表达,并且与淋巴结转移有显著相关性,但和性别、年龄、病理分型、临床分期无明显相关性。NF-κB/p65、MMP-3、MMP-9两两之间均呈正相关,三者在鼻咽癌的致病过程中可能存在协同作用。
Objective：To test the expression of NF-κB/p65,MMP-3 and MMP-9 in nasopharyngeal carcinoma （NPC）,and analyze their relationship and the clinical significance.Method：All samples were examined for the expression of NF-κB/p65,MMP-3 and MMP-9 by streptaividin-peroxidase immunohistochemistry.χ2-test and Spearman-test were used to exam the correlation within them and the expression difference between nasopharyngeal carcinoma and normal nasopharyngeal mucosa.Result：Positive expression rate of NF-κB/p65 in nasopharyngeal carcinoma was 78.0%; positive expressions rate of MMP-3 and MMP-9 in nasopharyngeal carcinoma were 75.6%. There was positive relationship between NF-κB/p65,MMP-3,MMP-9 and N stage. The expression of NF-κB/p65 showed a significant positive correlation with the expression of MMP-3 and MMP-9. MMP-3 also had a significant positive correlation with MMP-9.Conclusion：The result suggests that NF-κB/p65,MMP-3 and MMP-9 are highly expressed in nasopharyngeal carcinoma cells.Their expression is correlated with lymph nodes metastasis,but do not have relationship with sex,age,pathological classification and clinical stage. Each of them has positive correlation with the others.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2010年第15期678-682,共5页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词
鼻咽肿瘤
核因子-ΚB
基质金属蛋白酶
nasopharyngeal neoplasms
nuclear factor-kappa B
matrix metalloproteinase
