摘要
目的探讨系统性红斑狼疮(SLE)患者中Th1型细胞因子表达的情况。方法用逆转录 聚合酶链反应(RT PCR)法检测了SLE患者外周血单一核细胞(PBMC)中白介素2(IL 2)mRNA表达(41例)和γ干扰素(IFN γ)mRNA表达(31例)的水平。结果与正常人相比 ,活动期SLE患者中IL 2表达水平降低者占78 0 % ,IFN γ表达水平降低者占83 9 %。活动期SLE组PBMC中IL 2和IFN γmR NA表达的平均水平也降低(P均<0 01)。结论SLE患者中Th1型细胞因子的表达下调 。
Objective To investigate the mRNA expression of Th1 type cytokines in SLE patients Methods Applying RT-PCR technique to semiquantitatively analyse the IL 2 mRNA expression in PBMCs from 41 SLE patients and IFN γ mRNA expression in PBMCs from 31 SLE patients Results Compared with normal controls, the SLE patients with decreased expression of IL 2 accounted for 78 0% of the SLE patients in active stage,and those with decreased expression of IFN γ accounted for 83 9%, the mean levels of IL 2 and IFN γ expression in SLE group were significantly decreased as well (P< 0 01,both) Conclusion The Th1 type cytokine expression of SLE patients in active stage is down regulated,which may play a role in the pathogenesis of SLE
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
1999年第1期3-5,共3页
Chinese Journal of Dermatology
基金
高等院校博士学科点专项科研基金!(9502084)资助课题
关键词
红斑狼疮
系统性
TH1型细胞因子
RNA
信使
Lupus erythematosus, systemic Interleukin-2 Interferon typeⅡ RNA, messenger Polymerase chain reaction
