摘要
目的:探讨Th1/Th2细胞因子失衡与系统性红斑狼疮(SLE)发病的关系,为研究SLE的免疫发病机制提供新的思路,为SLE患者的临床诊断和治疗提供实验依据。方法:运用三色荧光标记法流式细胞术检测35例SLE患者(狼疮性肾炎组和不伴狼疮性肾炎组)和正常对照组20例外周血中Th1和Th2胞内细胞因子γ干扰素(IFN-γ)、白细胞介素-4(IL-4)的分泌情况。结果:(1)狼疮性肾炎(LN)患者Th1及Th1/Th2比值较对照组明显升高(P<0.01)。(2)不伴有LN的SLE患者Th1较对照组降低(P<0.05)。(3)LN患者Th1及Th1/Th2比值较不伴有LN的SLE患者明显升高(P<0.01)。结论:伴有LN的SLE患者Th1细胞功能亢进,具有Th1优势。不伴有LN的SLE是一种Th2细胞相对占优势的自身免疫性疾病。
Objectives：To discuss the relationship between Th1/Th2 cytokines imbalance and the cause of SLE,to provide new thoughts for studying immune pathogenesis of SLE ,to offer experimental proof for clinical diagnosis and therapy of SLE. Metbods： Three-color flow cytometric analysis was used to quantitate Th1/Th2 intracellular cytokines（IFN-γ and IL-4）from peripheral blood in 35 patients with SLE （lupus nephritis group and non-lupus nephritis group） and 20 healthy controls. Results：（1）Th1 and Thl/Th2 ratioes were significantly higher in the lupus nephritis group than that in the normal control（P〈0.01）. （2）Th1 was lower in the non-lupus nephritis group than that in the normal control（P〈0. 05）. （3）Th1 and Th1/Th2 ratioes were significantly higher in the lupus nephritis group than that in the non-lupus nephritis group（P〈0.01）. Conclusions：There was a Th1 predominance in lupus nephritis. SLE（non-lupus nephritis）is a relative Th2 predominance disease.
出处
《南通大学学报（医学版）》
2005年第4期253-254,共2页
Journal of Nantong University(Medical sciences)
