摘要
目的验证新发现的4个系统性红斑狼疮(SLE)相关基因与SLE发病的相关性,为SLE发病的分子机制研究提供资料。方法前期基因芯片工作中筛选得到4个在55例SLE患者及正常对照之间表达差异显著的基因,染色体定位发现其位于SLE连锁的染色体区段。应用TaqMan实时荧光定量聚合酶链反应(PCR)技术检测该4种基因在SLE患者和正常对照外周血单核细胞及T、B淋巴细胞的表达。结果SLE患者外周血单核细胞中,TRIM基因ΔCt值显著低于正常人对照(P＜0．05),基因表达水平为正常对照的0．61倍；IL-4R基因与EGR1基因的ΔCt值显著高于正常人对照(P＜0．05),基因表达水平分别为正常对照的2．62、2．03倍；KLRC1基因的ΔCt值显著高于正常人对照(P＜0．01),基因表达水平为正常对照的5．33倍。在SLE患者外周血T细胞,TRIM基因ΔCt值显著低于正常人对照(P＜0．01),基因表达水平为正常对照的0．56倍；IL-4R基因与EGR1基因的ΔCt值显著高于正常人对照(P＜0．05),基因表达水平分别为正常对照的2．55、1．74倍；KLRC1基因的ΔCt值显著高于正常人对照(P＜0．01),基因表达水平为正常对照的5．19倍。在SLE患者外周血B淋巴细胞,由于B细胞淋巴所占淋巴细胞比率过低,基因检测数据不稳定,故未作统计学分析。4种狼疮疾病相关基因与狼疮活动指数SLEDAI无相关性。结论SLE患者TRIM,IL-4R,KLRC1和EGR1基因与正常人相比有明显差异,但与疾病活动程度无关。
Objective To identify the relationship between the four systemic lupus erythemstosus （SLE）-related genes and the pathogenesis of SLE, provide information for SLE at molecule level. Methods Four genes, which expressed significantly different between SLE patients and healthy controls, were found by gene chips＇ screening. Chromosome location found that they were located at SLE linkage chromosome zone. TaqMan real time quantitative PCR was used for detecting KLRC1, TRIM, EGR1 and IL-4R mRNA expression in 55 SLE patients and 30 controls. Results In the peripheral blood mononuclear cells of SLE patients, △Ct of gene TRIM was significantly lower than healthy controls （P〈0.05）, the gene expression level was 0.61 times of that of normal controls, △Ct of genes IL-4R and EGR1 were significantly higher than those of healthy controls （P〈0.05）, the gene expression level was 2.62 and 2.03 times of those of normal controls respectively, △Ct of gene KLRC1 was significantly higher than that in healthy controls （P〈0.01）, the gene expression level was 5.33 times of normal controls. In the peripheral blood T cells of SLE patients＇, △Ct of gene TRIM was significantly lower than that of healthy controls （P〈0.01）, the gene expression level was 0.56 times of that of normal controls; △Ct of genes IL-4R and EGR1 were significantly higher than those of healthy controls （P〈 0.05）, the gene expression level was 2.55 and 1.74 times of those of normal controls respectively, △Ct of gene KLRC 1 was significantly higher than that of healthy controls （P〈0.01）, the gene expression level was 5.19 times of that of normal controls. Because the ratio of peripheral blood B lymphocytes in lymphocytes was so low, gene detection data was instable so statistical analysis was not carried out. There was no correlation between the four SLE-related genes and SLEDAI score. Conclusions The expression of TRIM, IL-4R, EGR1 and KLRC 1 genes in SLE patients are significantly different from those of healthy contro
出处
《中华风湿病学杂志》
CAS
CSCD
2006年第8期478-481,共4页
Chinese Journal of Rheumatology
基金
广东省自然科学基金(021339)
关键词
红斑狼疮
系统性
基因表达
聚合酶链反应
Lupus erythematosus, systemic
Gene expression
Polymerase chain reaction
