摘要
目的探讨支气管哮喘(哮喘)患者CC16与气道炎症及Th1/Th2细胞因子的关系。方法采取病例对照研究,收集25例哮喘急性发作期患者和33例健康对照组。采外周静脉血分离血浆,提取外周血单个核细胞(PBMC),用ELISA测定血浆中CC16、干扰素-γ(IFN-γ)、白介素-4(IL-4)的水平;用RT-PCR法检测PBMC转录因子T-bet和Gata-3mRNA表达水平。结果 1.哮喘组CC16及IFN-γ分别为(21.96±7.31)ng/ml,(118.73±22.59)pg/ml,明显低于对照组[分别为(64.88±25.27)ng/ml和(145.53±29.50)pg/ml,(均P<0.01)],哮喘组IL-4(425.22±4.37)pg/ml高于对照组(69.72±10.15)pg/ml,(P<0.01)。2.哮喘组T-betmRNA、T-bet/GATA-3表达水平(0.12±0.01,0.25±0.04)显著低于对照组(0.48±0.12,1.894±0.65)(均P<0.01),GATA-3mRNA表达(0.45±0.05)较对照组明显升高(0.30±0.08)(P<0.01)。3.CC16与T-betmRNA表达水平、T-bet/GATA-3呈正相关(r分别为0.792,0.761,均P<0.01);与GATA-3mRNA无明显相关性(r=-0.146,P=0.551)。结论 CC16参与哮喘气道炎症反应,并以Th1/Th2细胞因子失衡为特点。
Objective To detect the relation between CC16 and T-bet,GATA-3 of patients with asthma.Methods The plasma levels of CC16,IFN-γ and IL-4 were measured by enzyme-linked immunoadsorbent assay（ELISA）and the mRNA expression of T-bet and GATA-3 in the PBMC by reverse transcription-polymerase chain reaction（RT-PCR）from 25 exacerbating asthmatic group and 33 healthy control group.Results CC16 and IFN-γ in the asthma group were [（21.96±7.31）ng/ml,（118.73±22.59）pg/ml）] lower than that in the control group [（64.88±25.27）ng/ml,（145.53±29.50）pg/ml,respectively,all P〈0.01）],The IL-4 in the asthma group was（425.22±4.37）pg/ml significantly higher than that in the control group（69.72±10.15）pg/ml,（P〈0.01）.The expression of T-bet mRNA and T-bet/ GATA-3 ratio of PBMCs in asthma were significantly higher than that in the control group（all P〈0.01）;The expression of GATA-3 mRNA was significantly lower than that in the control group（P〈0.01）;The level of CC16 had positive correlation with T-bet mRNA expression and ratio of T-bet/GATA-3（r=0.792,r=0.761,respectively,all P〈0.01）.There was no correlation between CC16 with the expression GATA-3 mRNA（r=-0.146,P=0.551）.Conclusion The findings suggest that CC16 has close relationship with the airway inflammation and imbalance of Th1/ Th2 of asthma.
出处
《临床肺科杂志》
2010年第10期1419-1420,共2页
Journal of Clinical Pulmonary Medicine
