摘要
目的:观察针药结合治疗对局灶性脑缺血-再灌注大鼠模型脑组织SOD活性、MDA及NO含量的影响,探讨针药结合治疗对缺血性脑血管病病理状态的干预机制。方法:120只大鼠随机分为两组,分别于缺血2 h再灌注后1 h和再灌注后13 h进行治疗,并分别于再灌注后12 h(I2 h/R12 h组)和再灌注后1 d(I2 h/R1 d组)断头开颅取脑,检测脑组织SOD活性及MDA和NO含量。每组又分为4个亚组,分别是模型组、假手术对照组、头针组及针药组。除假手术对照组外,各组大鼠均采用线栓法建立局灶性脑缺血-再灌注模型,头针组以"百会"透"曲鬓"治疗,针药组以补阳还五汤灌胃结合"百会"透"曲鬓"治疗。结果:①在I2 h/R12 h组里,模型组大鼠脑组织SOD活性明显下降,MDA和NO含量显著升高;头针组和针药组大鼠脑组织SOD活性升高,MDA和NO含量降低。②在I2 h/R1 d组里,针药组MDA含量显著低于头针组。结论:对于脑缺血-再灌注模型鼠,头针及针药结合治疗可抑制自由基代谢及改善线粒体功能,减轻氧自由基介导的脂质过氧化反应。
Objective：To observe the effect of electric acupuncture combined with herbs on SOD activity and contents of MDA and NO in brain tissue of acute focal cerebral ischemia reperfusion model rats.Methods：120 rats were randomly di-vided into 2 groups（I2 h/R12 h group and I2 h/R1 d group）,then each group was divided into 4 groups,model group,sham operation group,electric acupuncture group,electric acupuncture combined with herbs group.Model rats were con-structed by suture method.The electric acupuncture group rats were punctured at Baihui point through Qubin point.SOD activity,contents of MDA and NO in brain tissue of rats were measured.Results： ①In I2 h/R12 h group,compared with sham operation group,SOD activity was decreased（P0.01）,contents of MDA and NO were increased significantly in mod-el subset（P0.05）;compared with model group,SOD activity was increased（P0.05）,contents of MDA and NO were de-creased in electric acupuncture group and electric acupuncture combined with herbs group.②In I2 h/R1 d group,MDA content in electric acupuncture combined with herbs group was significantly lower than that of electric acupuncture group.Conclusion：Traditional Chinese medicine therapy can restrain the production of free radicals,better the function of mito-chondria,alleviate lipid peroxidation induced by free radicals.
出处
《山西中医学院学报》
2010年第3期25-27,31,共4页
Journal of Shanxi College of Traditional Chinese Medicine
关键词
百会透曲鬓
补阳还五汤
局灶性脑缺血再灌注
自由基
Baihui point through Qubin point
Buyang Huanwu Decoction
acute focal cerebral ischemia-reperfusion（CIR）
free radicals
