摘要
目的 探讨电刺激小脑顶核 (FN)对局灶性脑缺血“治疗时间窗”的影响。方法 建立大鼠小脑FN电刺激及局灶性脑缺血再灌注 2 4h模型 ,应用尼氏体染色及神经元数量及形态分级评分法 ,观察电刺激FN对较长时间局灶性脑缺血 (3、6及 8h)后再灌注 2 4h ,缺血再灌注区感觉皮质神经元数量及形态改变的影响。结果 缺血 3、6及 8h后再灌注 2 4h ,神经元保存分别为 3 2 %±11 3% ,2 6 %± 4 5 %及 3 8%± 3 2 % ,评分均为 4 0± 0 0 ,各组之间差异无显著意义。而预先电刺激FN ,缺血 3h后再灌注 2 4h ,神经元保存 6 4 2 %± 11 3% ,评分明显降低 (2 1± 0 2 ) ,与相应时点对照组相比差异有显著意义 (P <0 0 1) ,但神经元明显缩小 ;缺血 6h后再灌注 2 4h ,部分神经元保存(32 8%± 6 5 % ) ,评分亦有所降低 (3 0± 0 0 ) ,与相应时点对照组相比差异有显著意义 (P <0 0 5 ) ,神经元形态呈缩小或不规则 ;缺血 8h后再灌注 2 4h ,神经元几乎完全丧失 (4 1%± 3 5 % ) ,评分为4 0± 0 0 ,与相应时点对照组相比差异无显著意义 (P >0 0 5 )。结论 电刺激FN可延长局灶性脑缺血“治疗时间窗” ;神经元的完全恢复可能仍需要其他进一步干预措施。
Objective To investigate the effect of cerebellar fastigial nucleus(FN) electrical stimulation on the therapeutic window of opportunity for intervention of focal cerebral ischemia. Methods Thirty-five healthy male Wistar rats were divided into focal cerebral ischemia/reperfusion group (I/R group, undergoing ischemia by embolism of middle cerebral artery for 3, 6 or 8 hours and then undergoing reperfusion for 24 hours, thus subdivided into I/R 3 hours, 6 hours, and 8 hours subgroups of 5 rats), focal cerebral ischemia/reperfusion plus FN electrical stimulation group (I/R-FN group, n=15, undergoing FN electrical stimulation followed by focal cerebral ischemia/reperfusion as in the I/R group), and sham operation group ( n =5). Twenty-four hours after the reperfusion or sham operation, the rats were killed. The brain slices underwent Nissl′s staining. Two slices of each rat were examined to observe the neuronal number and morphology, and the status or Nissl′s staining, and make a scoring of the affected somatosensory cortex. Results The survival rates of neurons of the I/R 3, 6 and 8 hours subgroups 3.2%±11.3%,2.6%±4.5% and 3.8%±3.2% respectively without a significant difference between any 2 subgroups (all P >0.05). The scores of these 3 subgroups all reached the highest grade (4.0±0.0). The neuron survival rate of the I/R FN 3 hours subgroup was 64.2%±11.3%, significantly higher than those or other I/R subgroup at the same time point (all P <0.01), however, the neurons being obviously shrunken. The score of the I/R FN 3 hours subgroup 2.1±0.2, significantly lower than that of the I/R 3 hours subgroup ( P <0.01). The neuron survival rate of the I/R FN 6 hours subgroup was 32.8%±6.5%, significantly higher than that of the I/R 6 hours subgroup ( P <0.05), however, the neurons being shrunken and irregular in shape. The score of the I/R FM subgroup was 3.0±0.0, significantly lower than that of the I/R 6 hours subgroup ( P <0.05). The I/R FN 8 hours subgroup showed a neuron survival rate of 4.1%±3.5%, not si
出处
《中华医学杂志》
CAS
CSCD
北大核心
2003年第13期1173-1175,共3页
National Medical Journal of China
