摘要
目的：通过各种诱变方法，获得一株ε-聚赖氨酸高产菌株。方法：以S．albulus KCTC 9669为出发菌株，经紫外（UV）、硫酸二甲酯（DMS）、硫酸二乙酯（DES）、亚硝基胍（NTG）的逐级诱变选育，最终获得一株稳定的高产突变株。结果：获得一株稳定高产的突变株N31-69，其摇瓶发酵产量为1．314g／L，比S.albulus KCTC 9669自然分离获得的诱变出发菌株SIPI-1产量提高11．8倍。结论：通过多种物理化学诱变，最终获得一株稳定的ε-聚赖氨酸高产菌株N31-69，为今后ε-聚赖氨酸产业化打下基础。
Objective：To obtain a high-yield strain of ε-polylysine through a variety of mutagenesis methods. Methods：Using S. a/bu/us KCTC 9669 as the initial strain, treated with UV,DMS,DES,NTG mutation to breed a stable and highyield strain. Results： A mutant N31-69 with the productivity of ε-polylysine 1. 314g/L in flask culture was obtained, which was increased by 11.8 folds in comparison with that of original strain SIPI-1. Conclusion：Through various physical and chemical mutagenesis, a stable and high-yield strain N31-69 can be obtained, which would pave a way for the industrialization of ε-polylysine.
出处
《赣南医学院学报》
2010年第3期356-359,共4页
JOURNAL OF GANNAN MEDICAL UNIVERSITY
基金
上海医药工业研究院自选课题,编号：SIPI2008-47
关键词
Ε-聚赖氨酸
诱变
选育
高产菌
ε-Polylysine
mutation
breeding
high-yield strain
