摘要
烟草 (Nicotiana tabacum L .)叶片在含有 2 mg/L 2 ,4 - D+0 .2 mg/L KT的 MS培养基上培养产生愈伤组织。用硫酸二乙酯 (DES)进行诱变处理 ,其半致死浓度为 0 .34%。处理后的愈伤组织接种到含有 15 %聚乙二醇 (PEG)的筛选培养基上 ,获得对水分胁迫具有抗性的愈伤组织 ,诱变率为 5 .4 %。抗性愈伤组织与对照相比 ,相对电导率和丙二醛 (MDA)
Callus of Tobacco(Nicotiana tabacum L.) was derived from leaves cultured on MS culture medium containing 2mg/L 2,4 D and 0.2 mg/L KT. Half death concentration of diethylsulfate (DES) which treatment for mutant inducement is 0.34%. When the treated callus inoculated on the selection medium contains 15% PEG, 5.4% of water stress resistant mutants were induced. Difference of the relative electrical conductivity and the content of malondialdehyde (MDA) between mutants and control reached very significant level.
出处
《种子》
CSCD
北大核心
2002年第3期26-27,30,共3页
Seed
关键词
组织培养技术
烟草
抗性
硫酸二乙酯
诱变
Tobacco Tissue culture Resistance Diethylsulfate Induced variation
