摘要
以甘蓝型油菜品种中双一号愈伤组织为材料，用ＥＭＳ、ＮａＮ３进行诱变处理．以草酸为选择剂进行了抗性筛选。经２％ＥＭＳ处理的愈伤组织扩增３代后，从８７２块愈伤组织中．筛选到７块抗（耐）３．５ｍｍｏｌ草酸的愈伤组织，其中有５块抗性稳定。抗性克隆的抗坏血酸氧化酶活性无甚变化，但多酚氧化酶以及过氧化物酶的酶活性都有显著的提高。
The calli cultures of Brassica napus cv. Zhongshuang No. 1 was treated with chemical mutagen and selection for oxalic acid resistant mutant was carried out. Results showed that suitable dosage of EMS (ethy1 methylene sulfonate) was 1 %～2% and the cultures was not sensitive to NaN3。From 872 clones asexually propagated 3 times from the callus treated with 2% EMS,7 clones resistant to 3. 5 mmol oxalic acid were screened out, and 5 out of 7 clones were stable in resistance. The activities of peroxidase and polyphenol oxidase were significantly higher than that of normal clones, but the activity of ascorbic acid oxidase did not change.
出处
《中国油料》
CAS
CSCD
北大核心
1996年第4期10-14,共5页
基金
国家"八五"油菜育种攻关课题
关键词
甘蓝型油菜
细胞诱变
草酸
突变体
Brassica napus Cell mutagenesis Oxalic acid Mutant
