摘要
目的观察浙江主产区栽培玄参的群体遗传结构。方法应用荧光AFLP标记对具有代表性的6个玄参居群群体遗传结构进行分析。结果采用7对AFLP引物组合扩增共得到12 552条扩增带,其中多态性谱带8 808条,多态性比率70.17%;各居群间的PPB变化范围在41.67%～55.56%,平均为47.30%;I在0.190 8～0.238 3,平均为0.221 8;Ne在1.201 4～1.280 6,平均为1.236 9;Gst为0.127 1,Nm为3.432 4;UPGMA聚类显示6个居群可以聚为2类,其中天台、缙云和景宁种群遗传距离较近聚为一个亚类,东阳、磐安和仙居聚为一个亚类。结论浙江主产区栽培玄参种质资源在物种水平上具有较高的遗传多样性,居群间有一定的遗传分化,但大部分存在于居群内部,居群间有较高的基因交流,居群间遗传距离与地理环境无相关性。
Objective To observe the genetic structure of cultivated Scrophularia ningpoensis in Zhejiang Province. Methods The genetic structures of six typical S. ningpoensis populations were analyzed by fluorescence AFLP marker. Results Bands （12 552） were generated by seven pairs of AFLP primer combinations, of which 8 808 were polymorphic, and the polymorphic rate was 70.17%. The variety ranges of PPB among different populations were 41.67%—55.56%, and 47.30% in average. I was between 0.190 8—0.238 3, and 0.221 8 in average. Ne was between 1.201 4—1.280 6, and 1.236 9 in average. Gst was 0.127 1, N m was 3.432 4. UPGMA Cluster analysis showed that the six populations can be divided into two clusters, as that of Tiantai, Jinyun, and Jingning were one sub cluster, and Dongyang, Pan′an, and Xianju were another one subcluster. Conclusion There is a relative high genetic diversity level in cultured S. ningpoensis of Zhejiang Province. Genetic differentiation exists among populations, but it exists in population mostly. There is a relative high genetic intercommunion among populations. The genetic distance is not related to the geographic environment.
出处
《中草药》
CAS
CSCD
北大核心
2010年第7期1160-1163,共4页
Chinese Traditional and Herbal Drugs
基金
温州市科技局资助项目(Y2004A121)
关键词
玄参
群体遗传结构
遗传多样性
AFLP
Scrophularia ningpoensis Hemsl.
genetic structures
genetic diversities
AFLP
