摘要
以厚皮甜瓜品种"银帝"为试材,根据黄瓜查儿酮合成酶(CHS)基因设计引物,运用半定量RT-PCR技术,研究了采后100mmol·L-1硅酸钠浸泡处理对甜瓜果实CHS基因表达的影响.结果表明:PCR扩增所得片段(494bp)与黄瓜CHS基因同源,相似性为94%,可用于半定量RT-PCR分析;CHS基因在处理和对照中的表达丰度极低,无法检测;损伤接种T.roseum后第4d,CHS基因大量表达,且硅酸钠处理+损伤接种与对照+损伤接种差异达到最大,前者高出后者12%.
The primer used in this experiment was designed according to the CHS gene from cucumber.Semi quantitative RT-PCR was used for evaluating the effect of sodium silicate dipping at 100 mmol·L^-1 on CHS gene expression of muskmelon fruit （Cucumis melon L. cv. Yindi）. The results showed that the PCR segments（494 bp）which shared 94 % homology with the cucumber CHS gene could be used for RT-PCR;the expression of CHS gene in the treated fruit and the control were too low to detect.However,an abundance expression of CHS gene was found 4 days after challenged with T.roseum,and 12 % higher in the treated fruit ＋ challenged than control ＋ challenged.
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2010年第3期48-51,共4页
Journal of Gansu Agricultural University
基金
国家自然科学基金资助项目(30671465)
关键词
厚皮甜瓜
硅酸钠
CHS基因
诱导表达
muskmelon fruit
sodium silicate
chalcone synthase gene （CHS）
induced expression
