摘要
本研究用生物信息学方法对石榴及其他19个物种CHS蛋白的系统进化和motif元件进行分析,重点预测分析石榴、巨桉和拟南芥CHS蛋白的理化性质、氨基酸组成、磷酸化位点和二级结构。结果表明,石榴与巨桉亲缘关系最近,与拟南芥最远。石榴、巨桉和拟南芥CHS蛋白由20种氨基酸组成,数量和比例存在差异,石榴和拟南芥中亮氨酸数量最多,巨桉中丙氨酸最多;存在丝氨酸、苏氨酸和酪氨酸3个磷酸化结合位点,多数发生在苏氨酸和丝氨酸位置上;二级结构由α-螺旋、延伸链、无规则卷曲和β-转角构成,α-螺旋和无规则卷曲占比较高。在20个物种CHS蛋白的motif元件中,石榴CHS蛋白motif数量和位置与巨桉完全一致,与大多数物种相似,与拟南芥差异较大。本研究结果可为深入研究CHS生物学功能及其调控石榴果实呈色的机理提供理论依据。
The phylogenetic and motif elements of pomegranate and other 19 cultivars of CHS proteins were analyzed by bioinformatics methods in the study. The physicochemical properties, amino acid composition, phosphorylation sites and second structure of CHS proteins were emphatically analyzed in Punica granatum, Eucalyptus grandis and Arabidopsis thaliana. The results showed that Punica granatum had the closest relationship with Eucalyptus grandis and the farthest relationship with Arabidopsis thaliana. There were twenty amino acids in the CHS proteins of Punica granatum, Eucalyptus grandis and Arabidopsis thaliana, and the number and proportion were different. The leucine number was the highest in Punica granatum and Eucalyptus grandis, and alanine number was the highest in Arabidopsis thaliana. There were phosphorylation binding sites of serine, threonine and tyrosine, respectively, most of them existed in threonine and serine. The secondary structure was consist of alpha helix, extended strand, random coil and beta-turn, in which,the proportion of alpha helix and random coil were higher. In the motif elements of CHS protein of twenty cultivars, the number and location of CHS protein motifs in Punica granatum were identical to Eucalyptus grandis, similar to most cultivars, and significantly different from Arabidopsis thaliana. The results could provide theoretical bases for the further study on biological functions of CHS and mechanism of regulating the coloration of pomegranate fruit.
作者
冯立娟
尹燕雷
杨雪梅
唐海霞
李英朋
鹿英
Feng Lijuan;Yin Yanlei;Yang Xuemei;Tang Haixia;Li Yingpeng;Lu Ying(Shandong Institute of Pomology, Taian 271000, China;Guan County Dianzi Forestry Station, Guanxian 252525, China;Shiheng Town Forestry and Fruit Station of Feicheng City, Feicheng 271612, China)
出处
《山东农业科学》
2019年第2期7-12,共6页
Shandong Agricultural Sciences
基金
山东省重点研发计划项目(2018GNC111009)
山东省农业科学院农业科技创新工程学科团队项目(CXGC2018E22)
山东省农业良种工程南种北繁项目(2017LZN023)
关键词
石榴
花青苷
查尔酮合成酶
生物信息学
Punica granatum
Anthocyanin
Chalcone synthase
Bioinformatics
