摘要
目的探讨热休克蛋白60(HSP60)和nm23蛋白在结肠癌及癌旁组织中的表达及其临床病理意义。方法采用免疫组化法分别检测HSP60和nm23蛋白在60例结肠癌、45例癌旁不典型增生及20例正常黏膜组织中的表达,并分析两者的表达水平与结肠癌临床病理因素的关系。结果 HSP60在结肠癌、癌旁不典型增生及正常黏膜组织中的阳性表达率依次降低,分别为78.3%、37.8%、20.0%;相反nm23蛋白的阳性表达率依次增高,分别为56.7%、83.2%、91.7%,两者在组间的表达差异均有统计学意义(P<0.01)。在癌组织中HSP60的表达与淋巴结转移有关(P<0.05),nm23蛋白表达与肿瘤浸润深度及淋巴结转移有关(P<0.05)。HSP60与nm23在结肠癌中的表达无相关性(P>0.05)。结论 HSP60及nm23蛋白在结肠癌组织中的表达状态与结肠癌发生发展有关,联合检测两者对结肠癌的预后判断具有重要意义。
Objective To investigate the expression and significance of heat shock protein 60（HSP60） and nm23 protein in colon carcinoma.Methods The protein expression of HSP 60 and nm23 were detected in 60 cases of colon carcinoma（CC）,45 cases of atypical hyperplasia adjacent to the tumor（AH） and 36 cases of normal colon mucosa（NCM） by immunohistochemistry respectively.Results The positive rate of HSP60 was significantly depressed ordinal in CC,AH and NCM which was 78.3%,37.8%,20.0%（all P〈0.01）.However,the positive rate of nm23 protein was significantly increased ordinal in CC,AH and NCM which was 56.7%,83.2%,91.7%（all P〈0.05）.HSP60 expression was correlated with lymph node metastasis（P〈0.05）.In addition,a relationship was found between the nm23 expression and the depth of tumor invasion and lymph node metastasis（P〈0.05）.There was no correlation between the expression of HSP60 and nm23 protein in colon carcinoma.Conclusions The expression of HSP60 and nm23 protein are associated with invasion and metastasis in colon carcinoma.United detection of HSP60 and nm23 protein may be used in the prognostic judgment of colon carcinoma.
出处
《山东医药》
CAS
北大核心
2010年第25期15-17,共3页
Shandong Medical Journal
