摘要
采用PCR技术从大豆品种吉农17基因组DNA中分离得到大豆种皮特异性启动子片段SCSP,长度约为1268bp。序列分析表明SCSP与报道序列同源性达97%以上。将其与GUS基因融合,构建植物表达载体pSCSP-GUS后,转化到EHA105根癌农杆菌中,通过根癌农杆菌(Agrobacterium tumefaciens)介导法转化烟草(Nicotiana tabacum)NC89。转基因植株的GUS活性检测表明,仅能在种皮检测到GUS活性,而在茎、叶和花等其它组织中都未检测到GUS活性,证实SCSP片段具有种皮特异性表达功能。
The promoter region of Seedcoat-specific gene was isolated from the genomic DNA of soybean Jilin 17 by PCR method,and obtained the promoter fragment SCSP,the length of the clone sequence is 1268bp.Sequence analysis indicated that this fragment had 97% homology compared with the reported promoters.The cloned promoter was fused to the GUS reporter gene to construct plant expression vector pSCSP-GUS,which was transferred into tobacco（Nicotiana tabacum） NC89 by Agrobacterium tumefaciens-mediated method and severa1 transformed plants were obtained.GUS activity assays of different soybean organs indicated that expression of GUS was active only in seedcoat,which suggests the SCSP gene is seedcoat-specific promoter.
出处
《大豆科学》
CAS
CSCD
北大核心
2010年第3期390-393,共4页
Soybean Science
基金
国家自然科学基金资助项目(30971805)
教育部博士点基金资助项目(20070193005)
关键词
大豆
种皮特异性启动子
功能分析
GUS染色
Soybean
Seedcoat specific promoter
Functional Identification
GUS staining
