摘要
实验构建了2个大豆贮藏蛋白Gy1基因种子特异性表达载体,并将其分别转入玉米愈伤组织中。载体1以潮霉素为筛选标记,将Gy1基因片段插入到含有种子特异启动子的植物表达载体pCAMBIA1301-7αP上,得到种子特异性表达载体pCAMBIA1301-7αP-Gy1;载体2以BADH为筛选标记,将Gy1基因插入到带有耐盐基因BADH的植物表达载体pCAMBIA1301-BADH上,得到安全性无抗生素选择标记的种子特异性表达载体pCAMBI-A1301-sbeⅡb-Gy1。通过农杆菌介导法将其分别导入玉米自交系H99的愈伤组织中,共获得132株再生植株;经过PCR检测,初步证明获得了34株转基因阳性植株。
Two seed-specificity expression vectors harboring seed storage protein Gy1 gene of soybean were constructed and the two vectors were transformed into embryogenic calluses of corn.Vector1,pCAMBIA1301-7αP-Gy1,was constructed by inserting Gy1 gene into pCAMBIA1301-7αP with selectable marker hyg,and vector 2,pCAMBIA1301-sbeⅡb-Gy1,was constructed by inserting Gy1 gene into pCAMBIA1301-BADH with salt-tolerance selectable marker BADH.The two vectors were transformed into embryogenic calluses of maize"H99"mediated ...
出处
《玉米科学》
CAS
CSCD
北大核心
2010年第2期23-26,共4页
Journal of Maize Sciences
